The electrophoretic patterns for 17 different cyanobacterial cultures derived from 6 different decamer primers were analysed to provide diagnostic fingerprints for each culture and their genetic distances based on RAPD markers.The primer OPB 09 produced a maximum of 24 amplified products. The primers OPB 09, OPG 04 and OPAH 02 generated markers specific for Nostoc cultures. Westiellopsis was found to be distinct from other cyanobacterial cultures in the RAPD profile obtained with the primer OPAH 02. The primer OPF 03 generated specific markers for Tolypothrix tenuis. Fischerella cultures could be identified with the primers OPB 09, OPAG 03 and OPF 05. The study revealed that these RAPD markers could be further used to identify and establish the genetic purity of the strains in the cyanobacterial inoculum. There was a similarity of 60-90% within Westiellopsis cultures. Nostoc cultures shared 50-80% similarity with Westiellopsis cultures. Anabaena cultures were similar to Westiellopsis cultures by 60-70%. The markers produced for each culture were also applied to phylogenetic analysis to infer genetic relatedness in this group of prokaryotes. The dendrogram analysis clearly revealed that free-living cyanobacterial cultures are closely related to each other and are distinct from the symbiotic forms.
Arbuscular Mycorrhizal Fungi is used as a potential factor in integrated plant protection. Mycelium of AMF functions as root hair and protects roots against soil borne pathogen (Azcón and Barea, 1997). It has been recognized that mycorrhizal symbiosis play a key role in nutrient cycling in the ecosystem and protects plant against environmental stress and plant diseases thereby improving the plant health (French,2017). The control of root rot diseases produced by fungi viz., Pythium, Pytophthora, Fusarium, Verticillium and Rhizoctoniais associated with AMF (Linderman, 1995). Also the increase in the absorption of nutrients, mainly phosphorus, supports the plant to withstand the attack of pathogenic microorganisms (Trotta et al.,1996). The interaction of the AM fungus Glomus fasiculatum with a wilt-causing soil borne pathogen F.oxysporum in cowpea
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