Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from a murine lupus strain, we have cloned a cDNA from the human T-cell line Many eucaryotic cells contain a cytoskeleton composed of 10-to 15-nm filaments and a karyoskeleton (nuclear envelope) made up of a meshlike lattice of 10-nm filaments. These structures, particularly the cytoplasmic filaments, are classically known as intermediate filaments (IF). IF proteins comprise a multigene family which may number 30 or more proteins in a mammalian species (for reviews, see references 3 and 61). This heterogeneous family comprises, at present, five principal types, of which the first four are tissue-specific cytoplasmic filaments. Type V IF proteins are the nuclear lamins, which have only recently been recognized as a distinct class of intermediate filament proteins (for reviews, see references 14 and 16).The lamins form a meshwork of filaments on the inner, or nucleoplasmic, surface of the nuclear envelope. but are no more closely related to each other than to lamin A and appear to be distinct lamins. LMDO, a lamin cloned and sequenced from Drosophila melanogaster (19), is larger than X. laevis L, but smaller than human and amphibian lamin A, and its sequence is sufficiently divergent to suggest that it is also a distinct lamin. Recently the X. laevis LI sequence was used to isolate a mouse lamin clone (FML11-1) whose protein sequence bears striking homology to the amphibian lamin LI (24). Although it lacks immunological identification, the acidic nature and distinct sequence of this lamin clearly distinguish it from mammalian lamins A and C and suggest that it is lamin B (24). Two acidic avian lamin proteins, B, and B2, have been described whose deduced amino acid sequences suggest that they represent structurally distinct B-type lamins (50, 68). However, whereas B1 appears immunologically related to mammalian lamin B, B2 is immunologically related to lamin A (37,64). It therefore appears that most nuclear lamins fall into two dominant but related types, A and B, which are themselves composed of subtypes.Although IF proteins form an insoluble skeleton within the cell, their functional significance is unclear. It is thought that they play some part in cellular differentiation (reviewed in reference 15). However, cell growth and division can proceed in the absence of most IF proteins, suggesting that their presence is not essential (23). The one exception to this appears to be lamin B, which is found in all somatic cells, even those lacking lamins A and C (20, 35, 62) and other IF proteins (48,49). This, together with the fact that the lamins appear to have arisen very early in eucaryote cell evolution (61), has led to the suggestion that the nuclear lamins, and particularly lamin B, may be the ancestors of the cytoplasmic IF proteins (14,61,69). At the least, it is apparent that the cytoplasmic ...
Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from a murine lupus strain, we have cloned a cDNA from the human T-cell line MOLT-4, which encodes nuclear lamin B. The identity of the encoded protein as lamin B was established by both biochemical and immunological criteria. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region. Analysis of mRNA in HL60 and MOLT-4 cells, which express only lamin B, or HeLa cells, which express all three major lamins (A, B, and C), together with the comigration of in vitro-translated product with isolated HeLa cell lamin B by two-dimensional gel electrophoresis, suggests that a single lamin B is expressed in mammalian somatic cells. In vitro translation with the cDNA clone revealed an EDTA-sensitive posttranslational modification which resulted in an increase in the apparent molecular weight to that equivalent to the native in vivo-synthesized lamin B protein. This in vitro modification included incorporation of a product of mevalonolactone and required an intact carboxy terminus.
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