Surface Sterilization of the plant material:The method most frequently utilized to detect and quantify endophytes involves isolation from surface-sterilized host plant tissues. Endophytic isolation was carried out under aseptic conditions. Different symptomless parts of the selected medicinal plants such as stem cuttings, leaves, and roots were used for the isolation of endophytes.The collected medicinal plant samples should be healthy and cleaned thoroughly in gentle running tap water AbstractMycoendophytes are microorganisms that reside internal tissues of living plants without causing any immediate overt negative effects, have been found in every plant species examined to date and recognized as the potential sources of novel natural products for exploitation in medicine, and agriculture with more bioactive natural products isolated from the microorganisms. In the past two decades, many valuable bioactive compounds with antimicrobial, insecticidal, cytotoxic, and anticancer activities have been successfully discovered from mycoendophytes. In the present investigation, endophytic fungi were screened form from stem, twig and leaves of Bixa orellanaL. collected form Sathyamangalam reserve forest. Predominant endophytic fungi isolated from the medicinal plantare Phyllosticta sp., Pestalotiopsis sp., and Phomopsis sp. Statistical analysis of Shannon Diversity Index, Jaccard Similarity Coefficient, and Endophytic Infection Rate were performed to understand the nature of mycophytes in the selected medicinal plant. This is the first report on endophytic fungi documented from this medicinal plant from Sathyamangalam forest of Tamil nadu, India.Keywords: Mycoendopytes; Jaccard similarity coefficient; Endophytic infection rate; Shannon diversity index
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