To investigate the mechanisms that human cytomegalovirus (HCMV) can vertically transmit from the placenta of mice to infect their offspring in the central nervous system (CNS) and cause congenital anomalies, and in order to provide basic research for preparing HCMV vaccine, we have developed a new type of mouse model of HCMV congenital CNS infection. Pure strain mice were propagated after being infected with HCMV. Then the degree of infection by HCMV to offspring was determined. The experiment shows that in the infection groups the mortality of fetal mice and the fatality of neonatal mice in one week are higher than that of the control groups (P < or = 0.05). At the same time we investigated the CNS of fetus's mice whose mothers were infected by HCMV. Our results showed: 1. The virus was successfully isolated from their cerebral cortex. 2. The signal of HCMV hybridization print was found in their nervous cell through in situ hybridization. 3. Especially human herpes virus-like particles and inclusion bodies in the plasm of nerve cell were found in the tissue of their brain under the electron microscope. This new type of mouse model of HCMV inherent CNS infection will help prepare HCMV vaccine and research HCMV congenital infection in CNS.
Objective: To campare the effectiveness of intramedullary and hemiarthroplasty in treatment of intertrochanteric fracture with coronal fracture in elderly Methods: A retrospective analysis of 50 elderly patients with intertrochanteric fracture with coronal fracture who performed operation between July 2017 and February 2022. According to the different surgical methods ,the patients divided into 2 groups: Group A(27 cases , performed with PFNA/Intertan) and Group B(23 cases, performed with hemiarthroplasy). There was no significant difference in age,gender,BMI,preoperative VAS score,preoperative hemoglobin and serum albumin levels,preopertive prepare time(P>0.05).Operation time, intraoperative blood loss ,length of hospital stay,postoperative VAS score,postoperative hemoglobin and serum albumin levels,complication,Harris score of postoperative 1,3,6 months were record and compared between the two groups. Results: There was no significant difference in operation time, length of hospital stay, postoperative hemoglobin and serum albumin level,complications between the two groups. There was significant difference in intraoperative blood loss between the two groups (P=0.001). The postoperative VAS scores of the two groups were no significant difference, but the postoperative VAS score were significant greater than preoperative in intra-group . Both of the two operations could effectively relieve pain. The Harris scores of the two groups were statistically significant at 1 month and 3 months after operation, and the function of hemiarthroplasty was better than that of intramedullary fixation, but there was no statistical significance between the two groups at 6 months. However, both surgical can significantly improve the function of patients. Conclusion: Both PFNA/Intertan intramedullary fixation and hemiarthroplasty can achieve good results for intertrochanteric fractures combined with coronal fractures. The existence of coronal fracture can increase the difficulty of operation. The early functional recovery of hemiarthroplasty is better than that of intramedullary fixation, but with the passage of time, intramedullary fixation can also achieve better function.
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