A procedure is described for the detection of five sulfonamides in muscle and kidney of swine, chicken and cattle. The method consists of an extraction with dichloromethane, cleaning up of the extracts on a Sep-Pak silica disposable column, and analysis by thin-layer chromatography on a silica plate with chloroform/n-butanol as an eluent. The sulfonamides were visualized by spraying with a fluorescamine solution. In spiked tissues the presence of the sulfonamides at concentrations of 0.05 mg/kg and higher is easily demonstrated. Direct detection of sulfonamides in tissue extracts of kidneys and lean tissues under the same chromatographic conditions is also described. This method, in which the Sep-Pak cleaning up procedure is omitted, has been successfully applied to spiked swine and cattle kidneys, chicken liver and breast tissue.
Aescoket | is an injection containing I[.54% (w/v) Ketamine Hydrochlorlde and 0.01% (w/v) Benzethonium Chloride in water. Both compounds could be analyzed by an HPLC system consisting of a silicagel column, dimensions 0.26 x 25 cm, particle size |0 ~m, a mobile phase of tetrabutylammonium bromide 0.[ M + dlsodlum hydrogen citrate 0.0[ M + water + methanol (i0 + 50 + 120 + 320, all by volume). Analysis of Ketamine was performed without sample clean-up; detection was performed at 219 nm. Results were compared with an assay according to Ketamine Hydrochloride injection USP XXI; the results correlated well. Benzethonium was extracted as an ion-pair with chloride into chloroform. After evaporation of the chloroform layer, the residue was redissolved in mobile phase and injected. Detection was performed at 225 nm. A stability study indicated an expiration date of two years after production by storage at room temperature.
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