In Indian Punjab, rice-wheat is a dominant cropping system in four agro-ecosystems, namely undulating subregion (zone 1), Piedmont alluvial plains (zone 2), central alluvial plains (zone 3), and southwestern alluvial plains (zone 4), varying in rainfall and temperature. Static and temporal variabilities in soil physical and chemical properties prevail because of alluvial parent material, management/tillage operations, and duration of rice-wheat rotation. A detailed survey was undertaken to study the long-term effect of rice-wheat rotation on soil physical (soil separates, bulk density, modulus of rupture, saturated and unsaturated hydraulic conductivities, soil water content, and suction relations) and chemical (organic carbon, pH, electrical conductivity) properties of different textured soils (sandy clay loam, loam, clay loam, and silty clay loam) in these four zones of Punjab. Soil samples (of 0-to 30-cm depth) from 45 sites were collected during 2006 and were analyzed for physical and chemical properties. The results showed that sand content and pH increased whereas silt and organic carbon decreased significantly from zones 1 to 4. Compared to other textures, significantly greater organic carbon, modulus of rupture, and pH in silty clay loam; greater bulk density in clay loam, and greater saturated hydraulic conductivity in sandy clay loam were observed. Irrespective of zone and soil texture, in the subsurface soil, there was a hard pan at 15-22.5 cm deep, which had high soil bulk density, modulus of rupture, more silt and clay contents (by 3-5%) and less organic carbon and hydraulic conductivity than the surface (0-15 cm) layer. These properties deteriorated with fineness of the soil texture and less organic carbon content. Continuous rice-wheat cropping had a deleterious 2945 effect on many soil properties. Many of these soils would benefit from the addition of organic matter, and crop yields may also be affected by the distinct hardpan that exists between 15 and 22.5 cm deep.