Saline antigen extracts of microfilariae, adult worms and third-stage larvae of subperiodic Brugia malayi maintained in gerbils were prepared for use as skin test reagents. Patients were studied on three different islands in the Philippines, one endemic for Bancroftian filariasis (Sorsogon, Luzon), another endemic for Malayan filariasis (Palawan) and the third without endemic filariasis (Cebu). A dose-response curve was established initially in patients with Bancroftian filariasis: thereafter 1.0 microng of the B. malayi antigens and 0.05 microng of Dirofilaria immitis FST antigen (obtained from Dr. T. Sawada) were used. Sizes of reactions were measured by recording the diameters of wheals at 20 minutes, 24 and 48 hours. There was a very high correlation in immediate hypersensitivity reactions among the three B. malayi antigens. Reaction sizes followed a normal distribution. When an area of an antigen-induced wheal 3 X that of the saline control was considered a positive reaction, 99% of 150 patients with Bancroftian filariasis and 96% of 45 subjects with Malayan filariasis reacted to B. malayi larval antigen. Only 68% of patients with Bancroftian filariasis but 90% of those with Malayan filariasis reacted to D. immitis FST antigen. There was no relationship between skin reactivity and age, sex, microfilaremia or severity of clinical disease. Approximately half of 50 patients who lived in an endemic area for W. bancrofti but had neither patent infection nor clinical disease reacted to B. malayi antigens. A maximum of 7% of 120 age- and sex-matched controls from Cebu gave false positive reactions with any of the antigens. Only a small proportion of patients gave 24- and 48-hour reactions. It is concluded that the use of antigens prepared from a human parasite, subperiodic B. malayi, which is easily maintained in a laboratory animal host, improves the ability to diagnose filarial infections by immunological means.
Unforeseen events can significantly affect organizations’ supply chains and disrupt their continuous flow. Therefore, organizations need to develop a response capability that allows them to minimize the negative effect of such events and quickly recover from them, also known as resilience. This research performs a comparative analysis of the influence that risk, vulnerability, and adaptability have on the resilience capability of supply chains in Colombian defense sector organizations before and during the coronavirus outbreak. Based on a literature review, a survey was designed and applied online to collect data from respondents related to the activities of the Colombian Air Force supply chain. For the first wave, data was collected between December 2019 and January 2020. Data for the second wave was collected in August 2020. Results suggest that identifying and managing risks positively impact reducing vulnerability and increasing adaptability. Moreover, by decreasing exposure and improving adaptability, the organization positively influences supply chain resilience capability. The results also indicate that the pandemic positively affected risk and vulnerability awareness. The identification of vulnerabilities had a positive impact on the resilience capacity during the Corona Virus outbreak. This research provides relevant information for the Colombian government on developing public policies and mechanisms of service and support for defense sector organizations to strengthen their resilience capability. Likewise, the study offers valuable information to those organizations interested in improving their resilience capability and that of the sector in which they are involved.
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