During the 2008-2010 period on light grey pseudopodzolic soils the following birdsfoot trefoil varieties and populations were studied: Shumen, Nesebar, Kiten, Tryavna, Sadovo, as well as genotypes of the varieties: V. Podolyanskii (Russia), Dedinovskii (Russia), Martanskii (Russia), Zora (Serbia), Bokor (Serbia), and Smolenskii (Russia). Variety Targovishte 1 (St) was used for comparison. The objective of the study was to investigate chemical composition, including content of crude protein, crude fiber, crude ash, with a view to growing those having the highest quality in the fore-mountainous conditions of the Central Northern Bulgaria. Crude protein content reached the maximum during the second year of growth. The highest crude protein value was measured in the forage of the varieties Bokor (193.1 g.kg -1 ) and Dedinovskii (190.2 g.kg -1 ). The mean value of crude protein in all studied varieties and populations was 129.8 g.kg-1, and the degree of variability was low (CV=9.78%). The crude fiber increased after the first year, and the differences of the mean values in the second and third year were not significant. The lowest fiber content in the three years was reported in the varieties Podolyanskii (225.8 g.kg -1 ), Martanskii (277.7 g.kg -1 ) and Targovishte 1 (288.1g.kg -1 ). The variation of crude fiber was highest in the third year, and CV values indicated a low the degree of variability (8.31%). The degree of variation of crude protein and crude fiber was approximately equal between the different accessions. It was found that, for the conditions of light grey pseudopodzolic soils on average for the period, variety Dedinovskii showed the highest crude protein content (160.3 g.kg -1 ) while variety Martanskii had the lowest percentage of crude fiber(288.1 g.kg -1 ). On average for the experimental period, maximum quantity of ash was contained in the forage of variety V. Podolyanskii -89.8 g.kg -1, and the mean value of ash was 81.3 g.kg -1 .
In 2014-2016 at the Research Institute of Mountain Stockbreeding and Agriculture-Troyan, we followed the change in the qualitative composition of dry biomass from two-component grass mixtures. The grasslands with the mixture of Trifolium repens L.-Poa pratensis L. have the highest crude protein content (151.6 g kg-1 DM), mineral substances (75.6 g kg-1 DM) and crude fat (33.9 g kg-1 DM). The legume grass associated with perennial ryegrass forms forage matter with the highest carbohydrate amount (434.9 g kg-1 DM) and the lowest amount of crude fiber (269.8 g kg-1 DM). The biomass of Lotus corniculatus L. and Festuca rubra L. is the richest in Ca (19.1 g kg-1 DM), and the mixture of blue hybrid alfalfa-cock's foot has the highest dry matter content (909.7 g kg-1 DM). The biomass of blue hybrid alfalfa-cock's foot (16.99 MJ/kg DM) and red clover-meadow fescue (16.96 MJ/kg DM) is with the highest caloric values. Both mixtures have almost identical values regardless of the different botanical composition and the predominant component in the grassland. The mixture of Trifolium pratense L. with Phleum pratense L. has the highest energy nutritional value (FUM-0.71 kg DM and FUG-0.66 kg DM), and the biomass of the blue hybrid alfalfa-cock's foot registered the lowest amount of exchange energy (7.30 MJ/kg DM), as well as the smallest number of feed units (FUM-0.67 kg DM and FUG-0.61 kg DM) in the dry matter. For the experimental period with the lowest values of gross energy (16.70 MJ/kg DM) are the mixed grasslands of Trifolium repens L.
Treatments of independent sowing of birdsfoot trefoil with organic mineral fertilizers were conducted during the period of 2010-2012 in the experimental field of RIMSA-Troyan to determine seed yield, structural elements contributing to yield, and correlation dependences among them. Biofertilizers included in the experiment consisted of: control-zero, phosphorus humate (3000 l/ha), boron humate (1600 l/ha) and molybdenum humate (1600 l/ha), as well as the combination of phosphorus humate (2500 l/ha) + boron humate (1000 l/ha) + molybdenum humate (1000 l/ha). It was determined that biofertilizers such as phosphorus humate, boron humate and molybdenum humate had a positive influence on the structural elements of the birdsfoot trefoil's seed yield. The highest effect from the tested fertilizers resulted from the combination of phosphorus humate, boron humate and molybdenum humate, as a result of which the number of seeds per racemes, the number of racemes per stem and the number of seeds in pod exceeded the control during the two years of the experimental period. The maximum average seed yield of 0.36 t.ha -1 using this fertilization combination demonstrated a direct relation between the yield and the structural elements. The boron and molybdenum humate provide a higher seed productivity in comparison with the independent fertilization with phosphorus humate. After treatment with these three biofertilizers, the seed yield is in positive correlation with all structural elements that define the seed yield. The correlation is manifested most strongly in relation to the number of pods per racemes and number of racemes per stem (r = 0.9291). The correlations between the yield and the number of pods per racemes and the number of racemes per stem are strong with positive values of the correlation ratio r = 0.6362 and r = 0.5012 respectively.
PURPOSE of the experiment is to study the influence of bird's-foot-trefoil cultivars of different origins over the productivity and quality of forage when they are grown together with Kenthucky bluegrass. METHODS: In the period of 2013-2015, in the experimental field of RIMSA-Troyan the productivity and botanical composition of bird's-foot-trefoil cultivars were studied, such as 'Targovishte 1', 'Delpon' (France), 'Fabio' (Italy), 'Haki' (Hungary), 'Vega' (USA) in two-component mixtures with Kenthucky bluegrass ('Sobra' cultivar). RESULTS: The share of sown species and cultivars in the mixed grasslands of bird's-foot-trefoil and Kenthucky bluegrass depends on their biological characteristics, combined with the climatic and soil characteristics of the area. 'Fabio' cultivar shows the most pronounced adaptability to the soil climatic conditions of the region compared to all other bird's-foot-trefoil cultivar. CONCLUSIONS: For the conditions of the Central Balkan Mountain, It is recommended to use the two-component mixture: bird's-foot-trefoil ('Delpon') and Kenthucky bluegrass ('Sobra'), which has the best intercompatibility and the most favourable combination of grass and legume component. The latter cultivar in the mixture recorded the highest average yields (650.37 kg/da dry weight) for the three-year study period, compared to other cultivars included in the experiment.
PURPOSE of the experiment was the impact of biofertilizers, such as boron and molybdenum humate on the chemical composition of grass biomass of bird's-foot trefoil, red clover and sainfoin. METHODS: The treatment of bird's feet, red clover, sainfoin with boron and molybdenum humate was studied at a dose of 1600 ml/ha. RESULTS: Boron humate had a positive impact on crude protein content in sainfoin. Molybdenum humate increased crude fats of bird's-foot-trefoil forage. Biofertilizers decreased crude fiber in sainfoin. Boron humate increased crude ash in red clover, while NFE had the highest values in bird's-foot-trefoil. Boron humate led to an increase in calcium content in bird's-foot-trefoil and sainfoin star, and decreased it in red clover. The developed graphical regression models: between plant height and the share of legumes in the grassland is y = 0.3232x + 17.709; crude protein and leaves y = 0. 1036x + 12.514; NFE and stems y = 0.1547x + 29.11; crude protein and calcium y = 2.9505x + 8.8375 allow the nutritional value of the feed to be predicted. CONCLUSIONS: Biofertilizers, such as boron and molybdenum humate (160 ml/da) can be recommended for inclusion in the growing technology for bird's-foot-trefoil, red clover and sainfoins, in order to improve forage quality.
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