Summary: A method for the detection of anabolic 17a-alkylated androstane derivatives in both plasma and urine is described and evaluated. The goat and rabbit antisera against 17a-Methyltestosteron-3-carboxymethyloxim-Rinderserum albumin were raised and compared using [ 3 H]methandrostenolone as a tracer. 22 Steroids including 10 potent synthetic anabolics were tested for their cross-reaction with these antisera.
Summary:A radioimmunoassay of nortestosterone and related steroids, including its principal metabolites, is described and evaluated. Antisera against nortestosterone-17|3-hemisuccinate-and nortestosterone-3-carboxymethyloxime-bovine serum albumin were raised in goats. By using a mixture of such antisera with different selectivity, the cross-reactions of several naturally occurring steroids can be reduced. The method can be applied for the detection of nortestosterone in both unprocessed or hydrolyzed urine extracts and also in plasma. It has been used as a screening test for anabolics iri doping control.
Radioimmunoassay fur Nortestosteron und verwandte SteroideZusammenfassung: Für die Bestimmung von Nortestosteron, seine Metabolite und verwandte synthetische aiiabole Steroide wurde ein Radioimmunoassay ausgearbeitet. Antisera gegen Nortestosteron-17]3-hemisuccinatund gegen Ngrtestosteron-3-carboxymethyloxim-Rinderserumalbumin wurden an Ziegen gewonnen und für die Bestimmung in einem optimalen Gemisch verwendet. Die Kreuzreäktion mit endogenen Steroiden kann auf diese Weise unterdrückt werden, so daß die Methode als ein Screeningtest für Anabolika vom Nortestosterontyp im nativen sowie hydrolysierten Harn und im Plasma für den Zweck der Dopingkontrolle verwendet werden kann.
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