Tentative identification using HPLC and RIA techniques indicated the presence of zeatin-0-glucoside, zeatin, ribosylzeatin, dihydrozeatin, iso-pentenyladenine and isopentenyladenosine in the petals of carnation flowers. Dihydrozeatin is apparently responsible for most of the biological activity.Within the petals most activity was detected in the basal parts which also senesced much slower than the upper parts of the petals. Treatment with AOA extended petal longevity and reduced ethylene production. This was associated with higher cytokinin-like activity in the basal parts of the petals.These higher levels of cytokinins were not observed in the petals of ACC treated flowers or in the detached control flowers. It is suggested that cytokinin transport and/or metabolism may play an important role in regulating ethylene production in cut carnations.
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