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Introduction: Traditional medicines for mammary tumour are unreasonable and have genuine symptoms. Non-ordinary normal medications have increased wide acknowledgment because of their assurance of a cure with negligible or no symptoms, however minimal experimental confirmation exists. One such basic cure is the leaves of the Euphorbia hirta plant. Methods: It is initially reported utilization of the fluid concentrate of Euphorbia hirta leaves breast cancer cells. The capacity of the concentrate to impel apoptosis and corruption in the human bosom malignancy cell line MCF-7, contrasted with typical human skin fibroblasts (MDA MB-231), was dictated by morphological changes in the cells utilizing light microscopy, DNA fragmentation, and brilliant stains (Annexin V and Propidium Iodide) utilizing Flow Cytometry and fluorescent microscopy. Results: Apoptosis was instigated in both cells, and more in MCF-7, when they were treated with 25% and half concentrate, while rot was watched mostly after presentation to raised concentrate fixations (75%). DNA discontinuity came about for both cells, in a period and dosage subordinate way. Both cells, at all concentrate fixations, demonstrated no critical contrasts in the quantity of living, dead, apoptotic, and necrotic cells. Conclusion: At long last, the outcomes might show that apoptotic changes in MCF-7 might be free of caspase-3, which is included in apoptosis and is inadequate in MCF-7 cells.

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Tafethion (ethion), an organophosphate insecticide was examined for its ability to induce chromosomal aberration and micronuclei (MN) in somatic (bone marrow) cells and sperm-shape abnormality in germ cells of mice tested in vivo.Mutagenicity of commercial preparation of ethion (tafethion 50 EC) was tested employing three doses (15, 22.5 and 30 mg/kg b.w.), three routes of adrtrlnistration (ip, po and sc), three hours of exposure acutdy (6, 24 and 48) as well as chronic (6 mg/kgfday; 5 days) for chromosomal aberration. All three doses were also tested for MN (30 hrs) and sperm-shape abnormality (35 days). The negative control was on glass double distilled (g.d.d.) water. The results in general, differed significantly from controls and indicated the mutagenic property of the chemical.

Silver nanoparticles (Hematite (α-Fe 2 O 3 ) have been used as an antimicrobial and as disinfectant. Nevertheless, there is limited data about its antitumor potential. This study focused on investigating the cytotoxic effects of Hematite (α-Fe 2 O 3 ) from Butea monosperma flower extract on MCF-7 breast cancer cells and its mechanism of action. Green method was created for the synthesis of Hematite (α-Fe 2 O 3 ) using an aqueous extract of B. monosperma flower. Synthesis of hematite (α-Fe 2 O 3 ) was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-[4, 5-dimethylthiazol-2-yl]-a 2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2',7'-dichlorofluorescein diacetate and intracellular calcium (Cai 2+ ) was evaluated with probe flu3-AM. Cells were treated with different concentrations of hematite (α-Fe 2 O 3 ) (1, 3, 6, 10, 15, 25, 50 and 100 μg/mL). The results showed that hematite (α-Fe 2 O 3 ) hindered cell growth in a dose-dependent manner. Hematite (α-Fe 2 O 3 ) appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai 2+ (IC 50 52 ± 3.14). In conclusion, the results of this preliminary study demonstrated that hematite (α-Fe 2 O 3 ) from B. monosperma flower extract may be a potential therapeutic potential medicament for human breast cancer treatment.

Among nanoparticles used for medical applications, Camellia Sinensis Nanoparticles (CSNPs) are among the least investigated. This study was undertaken to develop CSNPs by green synthesis using Camellia sinensis tea (Theaceae) plant extract to produce the NPs. The Camellia sinensis, Indian tea plant used from ancient time to increase appetite. Other medicinal uses have also been employed for the synthesis of super paramagnetic α Fe2O3 nanoparticles (NPs). The plant extracts revealed the phenolic groups bifunctional nature and capping nature through the –OH bonding over the nanoparticles (NPs) surface. The prepared nanoparticles (NPs) shows α-Fe2O3 phase among iron oxides and spherical morphology with an average size around 5 nm. The magnetic measurements proved the superparamagnetic behaviour of NPs with non-saturating MS value of 8.5 emu/g at room temperature (300 K). The CSNPs were characterized by UV-V is spectroscopy and X-ray Diffractometry, and evaluated with Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR). The CSNPs were spherical (size 7-20 nm) and contained phenols and flavonoids acquired from the Camellia sinensis extract. CSNPs has good 1-Diphenyl-2-Picrylhydrazyl (DPPH), OH, and NO scavenging properties. MTT assay showed that CSNPs (IC50 = 0.006 μM) were more antiproliferative toward the human MCF-7 cells than the Camellia sinensis tea extract (IC50 = 0.894 μM), Gemcitabin (IC50 = 2.133 μM). The anticancer cell effects of CSNPs on MCF 7 are mediated through the induction of apoptosis and G2/M cell-cycle arrest.

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