Fifty strains of red-pigmented, gram-positive, nonfermentative micrococci were studied, including organisms from diverse collections identified as Micrococcus roseus, M. agilis, "Sarcina erythromyxa, " "M. radiodurans, " "M. radiophilus, " and "M. radioproteolyticus" and miscellaneous unidentified strains usually labelled M. roseus (names in quotation marks are not on the Approved Lists of Bacterial Names, Int. J. Syst. Bacteriol. 30:225-420, 1980). Although similar in physiological attributes (negative characters predominated), the cell wall structural profiles separated M. roseus and M. agilis (simple homogeneous profile) from "M. radiodurans" and the radiation-resistant group (complex, multilayered profile). Simple reactions (growth in 5% NaCl broth, growth at 37OC, and nitrate reduction) distinguished M. roseus from M. agilis, and acid production from glucose and other sugars distinguished "M. radioproteolyticus" from the rest. The members of the "M. radiodurans" group could be typified by physiological reactions but not with great reliability. Gas-liquid chromatography of extracted lipids showed that veritable M. roseus and M. agilis strains had at least 50% of fatty acids in the form of 15:O branched chains. "M. radiodurans" and the rest had straight chains with a 16:l component which formed at least 25% of total fatty acids and which was not possessed by M. roseus or M . agilis. Further studies were based on representative strains of clusters derived from the abovementioned tests. Zymograms for nonspecific esterases and chromatograms of extracted pigments showed no identical patterns for any 2 of 10 strains. Absorption spectra for pigments had maxima at 450 to 510 nm. The guanine plus cytosine contents of the deoxyribonucleic acids ranged from 62 to 74 mol%: the M. roseus-M. agilis cluster was >69 mol%, and the radiation-resistant cluster was <71 mol%. There was little deoxyribonucleic acid homology between M. roseus and M. agilis
1. The antihypertensive neutral renomedullary lipid (ANRL) is a natural product derived from fresh renal medulla and from venous blood. 2. ANRL appears to be an antihypertensive hormone secreted when the kidney exerts its antihypertensive function after unclipping. 3. The kidney appears to be the main source of ANRL, maintaining a basal rate of secretion of ANRL. 4. The kidney of the one-kidney, one-clip hypertensive rat appears to secrete an inappropriate amount of ANRL. Thus a deficiency of the secretion of the antihypertensive hormone may play a role in the pathogenesis of the one-kidney, one-clip hypertensive model. 5. Degranulation of the renomedullary interstitial cells (RIC) occurs as the kidney exerts its antihypertensive action after unclipping, supporting these cells as the source of ANRL. 6. Channels between collecting duct cells may encourage water reabsorption while the clip is in place; conversely, the closure of these channels when the clip is removed may encourage the diuresis that is observed.
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