A new P32 labelled colloidal complex was introduced into the main trunk of the portal vein of the rabbit in order to examine its fixation and distribution within the liver. The dispersion percentage of the radiocolloid in the organism was minimal. Measurement of the radioactivity and autoradiohistographies would indicate a high stability of the preparation even some time after (2 months). Results are of particular significance when compared to those obtained with the introduction of the same radiocolloid into the general circulation.
Experimental results obtained with endosplenic injection of a new P32 labelled colloidal complex are reported. The measurement of the radioactivity, the autoradiohistography and the scintillografic finding obtained by a double labelling with P32 and Cr51 showed a high fixation and a homogeneous distribution of the radiocolloid within the liver. The application of the described method in the clinical field is suggested.
Abstract.
In order to study the influence of the development of a tumour on the leucopoiesis and on the life cycle of leucocytes, the incorporation of intraperitoneally administered 35S labelled cystine into the leucocytes of rats injected with Yoshida ascites sarcoma was investigated. The separation of leucocytes and the preparation of the samples were performed according to the method of Weisberger and Levine. Sulfur is incorporated in leucocyte proteins of rats injected with ascites tumour at a rate three times as high as in that of normal rats. The loss of 35S atoms from the leucocytes of tumour animals is more rapid, which suggests a shortening of the life span of W.B.C. due to the presence of the tumour. The striking similarity of these results with that obtained by Ehrenstein in the case of erythrocytes is emphasized. The possibility that the results obtained are due to the presence of tumour cells in the peripheral blood is discussed and ruled out.
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