This study studied the effect of combination pretreatment (ionic liquid choline acetate and NaOH) and pretreatment of NaOH in the process of delignification and lignocellulose crystallinity index. This process was carried out to obtain a good lignocellulose biomass, which has a low lignin content and a high crystallinity index to be prepared as a raw material for making bioethanol. Low lignin content and high index of crystallinity can influence the success of the enzymatic hydrolysis process and of course bioethanol production. In the combination of pretreatment, the highest lignin removal was found from process with 5% of NaOH as much as 41.63% but the cristalinity index was found from proces with 3% of NaOH as much as 46.33. While, In the NaOH pretreatment, the highest lignin removal and cristalinity index was found from process with 5% of NaOH as much as 7.63% and 3.87, respectively.
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