Fingermarks are one of the most useful forms of evidence in identification and can provide generalized proof of identity in crime investigation. They are developed using various conventional powders. The novel nanopowder ZnO-SiO2 was synthesized via the conventional heating method and characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), energy-dispersive X-ray (EDX) analysis, transmission electron microscope (TEM), and X-ray diffraction (XRD). The mean particle size of ZnO-SiO2 nanopowder calculated through TEM was 32.9 nm. The development of fingermarks was carried out by powder dusting and small particle reagent (SPR) methods. Powder dusting method was used for the development of latent fingermarks on various dry, nonporous, and semi-porous surfaces. The SPR method was also applied to wet nonporous surface. The developed latent fingermarks using ZnO-SiO2 nanopowder were found to have excellent quality with very clear third-level ridges detail and had better visibility than commercially available white powder.
Context.—The development of biotechnologic platforms capable of high throughput analysis has ushered in a promising new era of translational medicine. However, most studies to date are based on in vitro cell lines or substitute models for human disease. Although these model systems have proven insightful, it is readily becoming apparent that human clinical tissue must be studied in order to fully understand all the nuances of human disease. Studies that are based on human tissue, however, are limited by qualitative and quantitative issues, factors often precluding their use in high throughput studies. Objective.—To develop a simple and rapid tissue procurement protocol for use in obtaining a homogeneous epithelial cell population from clinical tissue and the recovery of nucleic acids and proteins of high quality and quantity. Also, to determine if the technique preserves tissue, thereby allowing morphologic correlation with molecular findings. Design.—Performance of manual exfoliation to procure cells from clinical resection specimens and use of immunomagnetic beads embedded with the antibody ber-Ep4 for the positive enrichment of a homogeneous epithelial cell population. Nucleic acids and proteins are then separated using a phenol plus guanidine thiocyante solution. Nucleic acids and proteins are quantitated and qualitatively analyzed using standard laboratory techniques. Results.—Nucleic acids and proteins of high quality and quantity were recovered following manual exfoliation and immunomagnetic bead separation. Tissue architecture was not destroyed, thus permitting histologic and molecular correlation. Conclusions.—A simple and reproducible protocol is presented that may enable the molecular profiling of clinically resected tissue. Although the technique is currently limited to certain tissue and tumor types, further research will broaden its overall application.
In the second trimester, fetal ventriculomegaly is a typical finding on obstetrical ultrasonography. It is clinically relevant since it can be caused by a variety of disorders that cause brain, motor, and/or cognitive impairment. Many cases are associated with other abnormalities, but in other cases, ventriculomegaly is the only abnormality. Objective: To evaluate the incidence of ventriculomegaly in 2nd and 3rd trimester with fetal outcomes. Methods: A descriptive study was conducted at of University of Lahore Ultrasound Clinic Greentown for duration of 4 months. Total 774 females were presented at hospital for in 2nd and 3rd trimester. All participants underwent transabdominal ultrasound using 3-5 MHz curvilinear probe. Written informed consent was obtained from all participants. Results: Total 774 women in 2nd and 3rd trimester of their pregnancy were included in our study. Out of 774 patients 700 were with cephalic presentation and 74 were with breech presentation. Variables including Spinal defect types, ventriculomegaly and ventricular involvement were noted. Out of 774 patients, 22 patients had fetal ventriculomegaly, 2 patients were with meningocele, 3 were with myelomeningocele and 16 patients were with spina bifida occluta. Ventricular involvement was as follow: In 5 fetuses, fourth ventricle was involved, in 15 fetuses lateral ventricles were involved and in 2 fetuses third ventricle was involved. Conclusion: Findings from this study concluded that Severity of ventriculomegaly is highly associated with fetal spinal defects.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.