Analyses of energy-resolved mass spectra (ERMS) of structural isomeric oligosaccharides were carried out under collision-induced dissociation conditions at different stages of MSn. Quantitative analyses of energy-resolved curves revealed that these curves can be used to distinguish anomeric configurations and such analysis can be made regardless of the stages of MSn. Furthermore, parameters obtained from the sigmoidal shaped curves obtained for ERMS were found to be used as the structural descriptors after statistical analysis. An important finding is that analyses of these descriptors depend on neither the m/z values nor the height factors.
Background: N-Glycan ␣2,6-sialylation regulates the cell surface residency of an anti-apoptotic molecule, platelet endothelial cell adhesion molecule (PECAM). Results: An ␣2,6-sialylated oligosaccharide inhibited the homophilic PECAM interaction and a cluster-type ␣2,6-sialyl N-glycan probe bound to PECAM-immobilized beads. Conclusion: PECAM is a weak sialic acid binding lectin. Significance: There is a possibility of using a glycan-based method to modulate angiogenesis.
Glycobiology has contributed tremendously to the discovery and characterization of cancer-related biomarkers containing glycans (i.e., glyco-biomarkers) and a more detailed understanding of cancer biology. It is now recognized that most chronic diseases involve some elements of chronic inflammation; these include cancer, Alzheimer's disease, and metabolic syndrome (including consequential diabetes mellitus and cardiovascular diseases). By extending the knowledge and experience of the glycobiology community regarding cancer biomarker discovery, we should be able to contribute to the discovery of diagnostic/prognostic glyco-biomarkers of other chronic diseases that involve chronic inflammation. Future integration of large-scale "omics"-type data (e.g., genomics, epigenomics, transcriptomics, proteomics, and glycomics) with computational model building, or a systems glycobiology approach, will facilitate such efforts.
The majority of structural investigations of oligosaccharides based on mass spectrometry use naturally occurring oligosaccharides, which do not allow extracting any common feature associated with anomeric structures and linkage positions. In order to address the issue to find such characteristics possibly contained in oligosaccharide structure, a synthetic combinatorial trisaccharide library was analyzed. The trisaccharides used in the analysis consisted of L-fucose, D-galactose and D-glucose, in which individual glycosidic linkages existed in either alpha- or beta-anomers. The analysis of energy-resolved mass spectra (ERMS) and the scattered plot analysis of some parameters obtained from ERMS for a series of trisaccharides revealed that lower activation energy was required for the dissociation of alpha-glycosides of these sugars compared to those of the corresponding beta-anomers. It is suggested that this finding may be useful in structural analysis of natural oligosaccharides.
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