The main objective of this study was to estimate the effect of supplementation with Saccharomyces cerevisiae (BIOSAF ® SC 47), on body condition score, milk production and some biochemical parameters in peripartum dairy cows. The experiment was conducted during 14 days before parturition to 45 days postpartum (20 milking). Holstein cows (16 primiparous and 4 multiparous) were randomly divided into two groups (n=10); the groups were fed with same diet: One group received, Saccharomyces cerevisiae. Milk production and blood samples were taken. Body condition scores (BCS) were recorded. For statistical analysis, Minitab ® 15.1.30.0.Fransh (© 2007 Minitab Inc) software was used. Addition of yeast culture to the diet ration was significantly increased. The average milk production per cow between the groups in all the experiment (by about +25%, i.e. 4.8 l/day/cow), with a positive effect in milk fat content and stabilization of the BCS, with reduction of reserve mobilization after calving. Moreover, this treatment has also induced systemic biochemical changes: increase of plasmatic total protein and albumin, whereas triglyceride and creatinine concentrations were significantly lowered, and cholesterol concentrations slightly reduced. The addition of yeast culture in the diet of Holstein cows was beneficial in improving milk production and milk fat, and some biochemical parameters.