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Food-based components represent major sources of functional bioactive compounds. Milk is a rich source of multiple bioactive peptides that not only help to fulfill consumers ‘nutritional requirements but also play a significant role in preventing several health disorders. Understanding the chemical composition of milk and its products is critical for producing consistent and high-quality dairy products and functional dairy ingredients. Over the last two decades, peptides have gained significant attention by scientific evidence for its beneficial health impacts besides their established nutrient value. Increasing awareness of essential milk proteins has facilitated the development of novel milk protein products that are progressively required for nutritional benefits. The need to better understand the beneficial effects of milk-protein derived peptides has, therefore, led to the development of analytical approaches for the isolation, separation and identification of bioactive peptides in complex dairy products. Continuous emphasis is on the biological function and nutritional characteristics of milk constituents using several powerful techniques, namely omics, model cell lines, gut microbiome analysis and imaging techniques. This review briefly describes the state-of-the-art approach of peptidomics and lipidomics profiling approaches for the identification and detection of milk-derived bioactive peptides while taking into account recent progress in their analysis and emphasizing the difficulty of analysis of these functional and endogenous peptides.

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Pre-sowing treatments viz, 50ppm GA 3, 2% CaCl 2, 0.5% KNO 3, Hydration (2h) and drying at room temperature <25 0 C and surface drying, and hydration-dehydration (2h) + Thiram dressing @ 0.25% were applied to two seed lots (marginal and good) of two cultivars of pigeonpea i.e. Paras and Manak and untreated seed lot was taken as control. The results revealed that various pre sowing invigouration treatments enhanced the standard germination of both the seed lots. However, the improvement in marginal seed lot (L 2 ) was more than good seed lot (L 1 ). Among the treatments, hydration and dehydration + thiram @ 0.25 percent were found most effective (6.0%) for enhancing in germination. Similarly, irrespective of the priming treatments among the cultivars Paras performed better under field conditions than Manak. irrespective of the cultivars, and seed lots pre-sowing seed treatments namely GA 3 (50ppm), cold hydration and cold hydration + 0.25% thiram showed improvement in germination and seed quality attributes, whereas remaining treatments showed negative effect. It was revealed also that variety Manak showed good enzyme activity (DHA, catalase, peroxide and superoxide dismutase) than the Paras. And among seed lots (Table 4) lot L 1 showed good enzyme activities than lot L 2 1

Image analysis is a state-of-the-art technique for seed quality testing. This tool provides vast usage in evaluation of various physiological and morphological characteristics of the seed with a more comprehensive perception. It is based on the extraction of numerical data from a captured image for characteristics like colour, size, shape of seed and seedlings and their subsequent processing with the help of suitable computer software. Speedy analyses, cost-effectiveness, automatic nature and user-friendly environment for work are some important advantages of Image Analysis over other conventional techniques. Numerous software have been developed for application in different fields of seed science research like germination studies, vigour estimation, varietal identification and purity testing etc. and most of these showed their potential adoption in the future as such or with some required transformations.

Global warming has increased the temperature significantly over a large extent both spatially and temporally. The threat of heat stress during the germination and seedling establishment stages in the wheat crop is now more prevalent than ever before. The present experiment assessed the effect of elevated temperature on the germination and early seedling growth of wheat genotypes. The seeds were sown under four temperatures, viz., 20 °C, 25 °C, 30 °C and 35 °C; the germination, seedling vigor and enzyme activities in 8-day-old seedlings were assessed. The temperature significantly influenced germination and early seedling growth. The germination percentage at 20 and 25 °C was statistically on par with and higher than at 30 and 35 °C. The seedling vigor parameters were maximum at 25 °C and showed a reduction at higher temperatures. Genotypic differences were observed for early heat stress as the genotypes WH 730, WH 1123 and HD 2967 showed tolerance towards heat stress during germination, whereas the genotypes PBW 725 and WH 1105 were susceptible. Antioxidant enzyme activities in seedlings increased with the rise in temperature. Catalase, peroxidase and superoxide dismutase enzymes showed increased activities at higher temperature levels.

Salt stress is one of the major constraints affecting plant growth and agricultural productivity worldwide. Sorghum is a valuable food source and a potential model for studying and better understanding the salt stress mechanics in the cereals and obtaining a more comprehensive knowledge of their cellular responses. Herein, we examined the effects of salinity on reserve mobilization, antioxidant potential, and expression analysis of starch synthesis genes. Our findings show that germination percentage is adversely affected by all salinity levels, more remarkably at 120 mM (36% reduction) and 140 mM NaCl (46% reduction) than in the control. Lipid peroxidation increased in salt-susceptible genotypes (PC-5: 2.88 and CSV 44F: 2.93 nmloe/g.FW), but not in tolerant genotypes. SSG 59-3 increased activities of α-amylase, and protease enzymes corroborated decreased starch and protein content, respectively. SSG 59-3 alleviated adverse effects of salinity by suppressing oxidative stress (H2O2) and stimulating enzymatic and non-enzymatic antioxidant activities (SOD, APX, CAT, POD, GR, and GPX), as well as protecting cell membrane integrity (MDA, electrolyte leakage). A significant increase (p ≤ 0.05) was also observed in SSG 59-3 with proline, ascorbic acid, and total carbohydrates. Among inorganic cations and anions, Na+, Cl−, and SO42− increased, whereas K+, Mg2+, and Ca2+ decreased significantly. SSG 59-3 had a less pronounced effect of excess Na+ ions on the gene expression of starch synthesis. Salinity also influenced Na+ ion efflux and maintained a lower cytosolic Na+/K+ ratio via concomitant upregulation of SbNHX-1 and SbVPPase-I ion transporter genes. Thus, we have highlighted that salinity physiologically and biochemically affect sorghum seedling growth. Based on these findings, we highlighted that SSG 59-3 performed better by retaining higher plant water status, antioxidant potential, and upregulation of ion transporter genes and starch synthesis, thereby alleviating stress, which may be augmented as genetic resources to establish sorghum cultivars with improved quality in saline soils.