Combinatorial adsorption–biodegradation treatment of textile wastewater provides a cost effective and ecofriendly alternative to conventional physicochemical treatment methods.
Plastid DNA markers sequencing and DNA fingerprinting approaches were used and compared for resolving molecular phylogeny of closely related, previously unexplored species of India. The utility of individual plastid markers namely, ,-, -, their combined dataset and two fingerprinting techniques viz. RAPD and ISSR were tested for their efficacy to resolves species into three sections specific clades namely, and. In the present study, sequences of these four plastid DNA regions as well as RAPD and ISSR profiles of 16 species together with six varieties of two species were generated and analyzed. Maximum likelihood and Bayesian Inference based construction of phylogenetic trees indicated that among the four plastid DNA regions tested individually and their combined dataset, was found best suited for resolving closely related species into section specific clades. When analyzed individually, exhibited better discrimination ability than ,-, - and combination of all four tested plastid markers. Among two fingerprinting techniques used, the resolution of species using RAPD was better than ISSR and combination of RAPD +ISSR and in congruence with resolution based on.
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