Muscle aging is a complex dynamic process that adversely affects whole‐animal metabolism and physiology. The molecular regulation of aging muscle that impacts muscle function, performance, and metabolism remains largely unknown because such studies in model organisms are prohibitively time‐ and resource‐intensive. The hawk moth, Manduca sexta, offers a unique opportunity to identify the genes that coordinate the muscle aging process. With a complete lifespan of about ten days and being similar to vertebrate muscle systems, this non‐vertebrate animal model is ideal to conduct a molecular dissection of the regulatory genes underpinning the senescence process in muscles. Here, we characterize the genome‐wide transcriptional changes by profiling gene expression from a high‐resolution time course sampling of muscle tissue from middle age to advanced age moths. Following RNA‐Sequencing of the time course tissues, we not only report differentially expressed genes across the time and day intervals, but also construct gene regulatory networks to identify candidate genes that direct the aging process. This approach will allow us to uncover novel interactions and generate new hypotheses as to the fundamental processes surrounding muscle aging. We will discuss the genes coordinating muscle senescence which include, but are not limited to, the down‐regulation of ribosome biogenesis, upregulation of Z‐band‐associated‐proteins, and down‐regulation of nicotinic acetylcholine receptors. Our results provide an unprecedented view into the complex molecular regulatory events orchestrating muscle senescence. Support or Funding Information South Dakota Board of Regents Competitive Research Grant #A20‐0013‐001; Nolop Institute of Medical Biology Summer Research Scholarship
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.