Our understanding of vascular morphogenesis and angiogenic growth has progressed considerably during the last few decades. Today the field has clarified the framework of interacting signaling pathways, intracellular regulatory genes and participating physical forces involved in the process of endothelial cell sprouting, lumen formation, and vascular remodeling. Nonetheless, once formed, vascular tubes also expand in width and length within the context of shear stress and tensional forces. Currently little information is available on how blood vessels perform this task or even what is the average half‐life of endothelial cells in the vascular wall. Using a combination of EdU incorporation, genetic tracing, rigorous quantification, and SILAM proteomics, our laboratory has gathered substantial information that sheds light into the cellular mechanisms and molecular players involved. The questions that will be addressed in this talk include: How does a vessel like the aorta expand in length and width while exposed to multiple physical forces? Do endothelial cells in an adult vessel divide to renew the endothelial lining or do [some] endothelial cells last for the lifetime of an individual? If endothelial cells proliferate: Is there a particular site/niche or do all cells all have equivalent capacity? Do endothelial cells have a finite number of cell divisions? What is the half‐life of endothelial cells in adult, normal blood vessels? What are the regulatory mechanisms involved in promoting vascular expansion? Taken together, we view this information as fundamental to our understanding the mechanisms controlling endothelial regeneration and their deregulations in settings like chronic/acute inflammation, aging, chronic diseases and physical trauma.Support or Funding InformationNIH/NHLBI R35HL140014This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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