Significant line-narrowing has been observed in the laser-induced fluorescence spectra of naphthalene and perylene in direct supersonic expansions of simple supercritical carriers such as CO2, N2O, and even SF6, under conditions which minimize aerosol and cluster formation. Clusters form readily in expansions of CO2 and N2O unless nozzle temperatures exceed about 200°C. Results with more complex carriers, such as n-pentane, are much poorer. Selective detection of perylene at a limit of 2 ng (S/N = 3) in a mixture of ten polycyclic aromatic hydrocarbons and in a coal tar extract has been achieved with a capillary introduction system. Challenges with coupling supersonic jet detection to capillary supercritical fluid chromatography lie with the nozzle.
Here, the full genome sequences of 22 T1-like bacteriophages isolated from wastewater are reported. Eight (BlueShadow, Brooksby, Devorator, ElisaCorrea, Reinasaurus, SorkZaugg, Supreme284, ZeroToHero) were isolated on
Citrobacter
, six on
Klebsiella
(Chell, FairDinkum, HazelMika, Opt-817, P528, PeteCarol), and eight on
Escherichia
(Fulano1, Mishu, Opt-719, PhleaSolo, Punny, Poky, Phunderstruck, Sadiya).
We announce the complete genome sequences of 14
Serratia
bacteriophages isolated from wastewater treatment plants. These phages define two previously undescribed types which we call the Carrot-like phage cluster (phages Carrot, BigDog, LittleDog, Niamh, Opt-148, Opt-169, PhooPhighters, Rovert, Serratianator, Stoker, Swain, and Ulliraptor) and Tlacuache-like phage cluster (Tlacuache and Opt-155).
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