ResumoA criação de pacas (Cuniculus paca Linnaeus, 1766) vem se desenvolvendo em toda América Latina. Com o objetivo de acompanhar o manejo sanitário, determinar a prevalência de parasitas gastrointestinais de pacas criadas em cativeiro, sua carga parasitária e avaliar estratégias utilizadas em seus controles, entre os meses de novembro/2012 a junho/2013, amostras de fezes frescas no solo foram coletadas e analisadas, utilizando-se as técnicas de Willis Mollay e Hoffmann. Para quantificação, utilizou-se a câmera de McMaster. A leitura foi realizada em microscopia óptica, com objetivas de 25x e 40x, as quais revelaram 21.433 ovos por grama de fezes (OPG), com média de 2.679,12 (± 41,0) Strongyloides spp., e 3.325 OPG, com média de 415,6 (± 57,0) Trychuris spp. Também encontraram-se 482.101 oocistos por grama de fezes (OOPG), com média 60.263 (± 15,93) de Eimeria spp. e 51 larvas de Strongyloides spp., com média 6,3 (± 6,86) larvas. Em uma única baia foram identificados dois ovos de Hymenolepis diminuta. Foi utilizado um anti-helmíntico (albendazol 10%) e trimetropina associada à sulfadiazina, para combater a eimeriose. Conclui-se que o grande índice de infestação parasitária apresentado pelos animais, mesmo a despeito dos tratamentos antihelmínticos efetuados, pode estar associado com o índice pluviométrico apresentado, as condições físicas das instalações e a limpeza e higienização dos recintos de forma inadequada. Palavras-chave: animal silvestre; nematoides; paca. Abstract Paca (Cuniculus paca Linnaeus, 1766) rearing has shown development in all Latin America countries. Fresh feces samples were collected from the soil and analyzed by Willis Mollay and Hoffmann's techniques, aiming at accompanying the sanitary management, determining the prevalence of gastrointestinal parasites, and their respective load, of pacas raised in captivity, and evaluating parasite control strategies, between the months of November/2012 and June/2013. A McMaster's camera was used for quantification. The reading was accomplished by optical microscopy with 25x and 40x lenses, which revealed 21,433 eggs per gram of feces (EPG), with an average of 2,679,12 (± 41.0) Strongyloides spp., and 3,325 EPG, with average of 415.6 (± 57.0) Trychuris spp. Besides, 482,101 oocytes per gram of feces (OPG) were found, with an average of 60,263 (± 15.93) Eimeria spp., and 51 larvae of Strongyloides spp., with an average of 6.3 (± 6.86) larvae. Two eggs of Hymenolepis diminuta were identified in only one stall. An anthelminthic (albendazole 10%) and trimethopim
Morphology, morphometry, and membrane integrity of epididymal spermatozoa of spotted pacas (Cuniculus paca, Linnaeus 1766)Morfologia, morfometria e integridade da membrana de espermatozoides epididimários de paca (Cuniculus paca, Linnaeus 1766) Highlights: Majority (73%) of the epididymal spermatozoa of Cuniculus paca were normal. Proximal cytoplasmic droplet was the second most frequent defect found. Both ACP-123 ® and Botusemen Special ® were efficient extenders. Intra-acrosomal vesicles were observed in the sperm head. AbstractThe objective of this study was to evaluate the morphology, morphometry, and membrane integrity of epididymal spermatozoa of spotted pacas using spermatic cells collected from the epididymal tails of five animals. The flotation method using the ACP-123 ® and Botusemen Special ® extenders was performed, and samples were stained in Diff-Quick and eosin-nigrosine. Descriptive statistics of data were obtained and Student's t-test was performed. The morphology of 200 Diff-Quick-stained spermatozoa showed that they had an oval head with three vesicles in the acrosomal region, a midpiece, an elongated tail; moreover, 27% of the spermatozoa exhibited cellular defects. The morphometry of 100 sperm cells (analyzed with an optical microscope and the EZ Leica LAS software for Windows) presented the following measurements (mean ± SD): total length 43.87 ± 4.91 μm, head 7.54 ± 0.82 μm, midpiece 5.35 ± 0.83 μm, tail 30.72 ± 2.55 μm, and head width 5.30 ± 0.68 μm. Of the 2,000 cells stained with eosin-nigrosine for membrane integrity evaluation, 83.8% diluted in ACP-123 ® and 72.9% diluted in Botusemen ® had intact membranes. The results of this study suggest that epididymal spermatozoa of pacas can be used in assisted reproduction programs; moreover, our study adds knowledge to the reproductive biology of wild animals, and encourages further research on the role of the three acrosomal vesicles present in this species. ResumoCom o objetivo de avaliar a morfologia, a morfometria e a integridade de membrana dos espermatozoides epididimários de paca, células espermáticas oriundas da cauda do epidídimo foram obtidas de cinco animais pelo método de flutuação, utilizando os diluidores ACP-123 ® e Botusemen Special ® , coradas em Panótico rápido e Eosina-Nigrosina. Os dados foram analisados por estatística descritiva e teste t de Student. A morfologia de 200 espermatozoides corados em Panótico rápido evidenciou que os mesmos possuíam: cabeça ovalada com três vesículas na região acrossomal, peça intermediária, cauda alongada, sendo os defeitos celulares de 27%. A morfometria de 100 células espermáticas (efetuada com microscópio óptico e softwares EZ Leica LAS de aquisição de imagens para sistemas operacionais Windows) apresentou as seguintes medidas (média ± d.p): comprimento total 43,87 ± 4,91 µm, cabeça 7,54 ± 0,82 µm, peça intermediária 5,35 ± 0,83 µm, cauda 30,72 ± 2,55 µm e largura da cabeça 5,30 ± 0,68 µm. Das 2.000 células coradas em Eosina-Nigrosina para avaliação da integridade da membrana, 83,8 % das ...
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