Aflatoxins are the main mycotoxin related to the contamination of Brazil nut and an important product extracted from the Amazon region that has a high number of consumers due to its health benefits. Considering the frequent occurrence of aflatoxins in Brazil nuts, it is necessary to study the levels of exposure to these toxins associated with its consumption using biomarkers. To do this, a study was carried out with 30 volunteers of both sexes, where each volunteer received a kit containing Brazil nuts for the ingestion of 2 (two) units / day for 30 days. Urine samples were collected from each volunteer at 0 and 30 days, and the level of Aflatoxin M1 (AFM1) was determined by ELISA using the Helica Kit®. A questionnaire was used to obtain information on weight, height, age, medication use, pathological conditions, frequency of weekly consumption of certain foods more susceptible to aflatoxin contamination, and 24-h food recall. Of the 30 samples analyzed at time 0 (zero) for exposure, AFM1 presented positive results in 3 (10%) samples with a range of 2.75-70.41 ng/mL. In the analysis of the 30 days after Brazil nut consumption, the presence of AFM1 in the urine was not detected, suggesting that Brazil nut consumption did not generate impact on the urinary levels and exposure to AFM1. In conclusion, no association was found between food consumption of food susceptible to aflatoxin contamination and concentration of AFM1 in urine.
Some conservation methods can add value to the product involved, especially those that involve meat derivatives. To take advantage of the raw materials available in the Amazon region, a semi-preserve using the meat of black caiman (Melanosuchus niger) was elaborated and evaluated for 90 days. The product was prepared using a curing brine of 4% acetic acid and 5% sodium chloride (NaCl), tail meat and back strap (sirloin), and was maintained at a controlled temperature of 5 °C for 30 d., with subsequent addition of flavoring brine (olive oil or Brazil nut oil), seasoning and soy lecithin for emulsification. The product was pasteurized at 70 °C/20 min and stored at 25 °C/90 d. The proximate composition, pH, acidity index, total volatile base nitrogen (TVBN), were determined. In the microbiological analysis for Salmonella sp., total coliforms and E. coli were within the standards required by current Brazilian legislation. The results of the physical-chemical analyses showed that the product had remained stable during the 90 d. of storage at room temperature (25 °C), and the pH values confirm that the immersion in acetic acid and sodium chloride in the proposed concentrations were enough to promote the reduction of pH and thus maintain the stability of the product.
Aflatoxins (AFL) are fungal metabolites that can contaminate animal feed, causing toxic effects after consumption. Among the AFL, there are reports of the occurrence of aflatoxin M1 (AFM1) in milk, so the objective of this work was to evaluate the AFM1 content in bovine milk samples produced in the State of Amazonas-Brazil. Of the analyzed samples (n=30), 20% were found to be contaminated with AFM1 in the range of 0.093-0.320 μg/kg (Limit of detection= 0.0625 μg/mL). No sample exceeded current Brazilian legislation, but all exceeded the maximum European regulatory limit of 0.05 μg/kg. Risk analysis estimated a daily intake of 5.357 x 10-7 mg/kg body weight/day for men and 6.257 x 10-7 mg/kg body weight/day for women. The MOE result did not indicate concern in public health aspects. On the other hand, the occurrence of AFM1 in the state of Amazonas was observed in an unprecedented way and need further investigation including other dairy products.
Introduction: The hygienic and sanitary conditions of vessels carrying passengers and cargo requires constant diagnosis and monitoring for disease prevention. Objective: The objective of this study was to identify health hazards in food preparation conditions and water potability in mixed vessels in the State of Amazonas-Brazil. Method: 33 vessels were analyzed with qualitative detection analysis of Escherichia coli by chromogenic substrate for water potability and environmental evaluation with questionnaire application. Results: There was microbiological contamination in 13 (39.39%) drinking water samples and 31 (93.93%) sink samples for E. coli. The structural and operational conditions of kitchen handlers mostly did not meet the minimum percentages of safety in food production. Conclusions: This is the first report on the structural and operational conditions of food preparation in mixed vessels in Manaus-AM and we conclude that intervention is necessary with public policies regarding hygienic and sanitary issues involving the supply of water served in vessels for the protection of passenger health and training of food handlers and inspection of environments.
A study was carried out to elaborate and monitor the shelf life of a pineapple jam with ingredients using, as “gelatinizing agent”, the pectin extracted from passion fruit residues from a rural community of Amazonas State, Brazil. The product was always maintained at a temperature of 25 °C and studied for 4 months, with the results showing physical-chemical and microbiological stability. We concluded that passion fruit residue can be used in pineapple jam production while still meeting legal standards for commercialization, reducing environmental waste, and being an alternative source of income in rural communities.
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