The Abdominal Aortic Aneurysm (AAA) is a silent and often deadly vascular disease
caused by the localized weakening of the arterial wall. Previous work has shown that local
changes in wall stiffness can be detected with Pulse Wave Imaging (PWI), which is a
noninvasive technique for tracking the propagation of pulse waves along the aorta at high
spatial and temporal resolutions. This study aims at assessing the capability of PWI to
monitor and stage AAA progression in a murine model of the disease. ApoE/TIMP-1 knockout
mice (N = 18) were given angiotensin II for 30 days via
subcutaneously implanted osmotic pumps. The suprarenal sections of the abdominal aortas
were imaged every 2-3 days after implantation using a 30 MHz Visualsonics Vevo 770 with
115 μm lateral resolution. Pulse wave propagation was monitored
at an effective frame rate of 8 kHz by using retrospective electrocardiogram (ECG) gating
and by performing 1-D cross-correlation on the radio-frequency (RF) signals to obtain the
displacements induced by the waves. In normal aortas, the pulse waves propagated at
constant velocities (2.8±0. 9 m/s, r2 =
0.89±0.11), indicating that the composition of these vessels was relatively
homogeneous. In the mice that developed AAAs (N = 10), the wave
speeds in the aneurysm sac were 45% lower (1.6±0.6 m/s) and were more
variable (r2 = 0.66±0.23). Moreover, the
wave-induced wall displacements were at least 80% lower within the sacs compared
to the surrounding vessel. Finally, in mice that developed fissures (N
= 5) or ruptures (N = 3) at the sites of their AAA,
higher displacements directed out of the lumen and with no discernible wave pattern
(r2 < 0.20) were observed throughout the cardiac
cycle. These findings show that PWI can be used to distinguish normal murine aortas from
aneurysmal, fissured, and ruptured ones. Hence, PWI could potentially be used to monitor
and stage human aneurysms by providing information complementary to standard B-modes.
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