Fermentation technology has been used for decades for dairy and meat products. Several studies have shown an increase in the nutritional value of fermented meat and can increase its safety and shelf life. Bekamal is a traditional fermented meat product found in Banyuwangi Regency. Lactic acid bacteria is one of the natural microbiota of meat that is widely used as a fermentation agent to maintain quality and improve the product’s hygiene and sensory properties. This study aimed to indetify lactic acid bacteria (LAB) from traditional fermented meat products, namely bekamal. Bekamal tested is meat that has undergone a fermentation process for 7, 14 and 21 days, and each fermentation time consists of one sample to be isolated and identified the presence of lactic acid bacteria in it. The results showed that in bekamal, some lactic acid bacteria were indicated by Gram staining results, namely gram-positive and catalase-positive, and the results of the CO2 production test were classified as heterofermentative bacteria. This research concluded that in bekamal, lactic acid bacteria were indicated from Lactobacillus, Streptococcus and Leuconostoc genus.
The content of protein and nutrients in beef makes microorganisms able to live and breed well, it is easy for pathogenic microorganisms to grow and make beef quickly damaged, so processing technology is needed to extend the shelf life such as fermentation techniques. Bekamal is a fermented beef product from the Banyuwangi district. Bacteria in the bekamal fermentation process are lactic acid bacteria. B14A isolate is assumed as lactic acid bacteria that isolated from bekamal which will be used as a starter for meat fermentation in this study. The purpose of this study is to determine the content of pathogenic microorganisms in fermented bekamal meat using B14A isolate. The samples used in this study were fresh beef and bekamal which was fermented by B14A isolate in 0, 7, 14, and 21 days and the commercial one for 1 month, 2 months, and 3 months. Parameters analyzed include the content of Escherichia coli, Staphylococcus aureus, and Salmonella sp. The method in this study was tested using Qualitative Descriptive Analysis with 8 samples and each consisting of 3 replications. The results showed that all samples of fresh beef were contaminated with three types of pathogens and all samples of bekamal were negative for all three types of pathogens.
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