The taxonomic status of two bacterial strains isolated from human blood was characterized using a polyphasic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus Williamsia and that produce C 16 : 0 and C 18 : 0 fatty acids on pyrolysis GC, and dihydrogenated menaquinone with nine isoprene units as the predominant isoprenologue. The generic assignment was confirmed by 16S rRNA gene sequencing. Comparative analysis of the 16S rRNA gene sequence showed that these isolates constitute a distinct phyletic line within the genus Williamsia, displaying 96?2 and 97?2 % sequence similarities to Williamsia muralis and Williamsia maris, respectively. The two isolates could be distinguished from the type strains of the latter species on the basis of several phenotypic traits. The genotypic and phenotypic data show that the strains merit classification as a novel species of Williamsia, for which the name Williamsia deligens sp. nov. is proposed, with type strain IMMIB RIV-956The genus Williamsia was proposed by Kämpfer et al. (1999) to accommodate actinomycetes with atypical cell morphology as revealed under electron microscopy that are unable to grow at 5 or at 45 uC and possess mycolic acids with carbon chain lengths of 50 to 56. Based on its mycolic acids, it seems that Williamsia takes an intermediate position between Rhodococcus (mycolic acid chain lengths of 34-45) and Gordonia (mycolic acid chain lengths of 54-66) (Kämpfer et al., 1999). The genus Williamsia currently comprises two recognized species, Williamsia muralis isolated from indoor building material of a children's day-care centre in Finland (Kämpfer et al., 1999) and Williamsia maris isolated from deep sediments of the Sea of Japan (Stach et al., 2004). In this paper we describe two bacterial strains which were isolated from human blood. Based on phylogenetic and phenotypic data it is proposed that these strains (designated IMMIB RIV-956 T and IMMIB RIV956Fl) are similar and should be classified as representing a novel species of the genus Williamsia.
Isolates IMMIB RIV-956T and IMMIB RIV-956Fl were isolated from human blood. The type strains of W. maris (DSM 44693 T ) and W. muralis (DSM 44343 T ) were received from the DSMZ. All strains were cultured on Columbia agar supplemented with 5 % sheep blood agar and brain heart infusion (BHI) agar to determine their morphological characteristics. Production of pigments was determined by growing the strains at 27 u C for 7 days, and observations were made at 24 h intervals. Air-dried smears at 24, 48 and 72 h intervals were stained by using the Gram's method in order to determine the Gram reaction and cell morphology.
A bacterial isolate obtained from soil from a municipal landfill site in India was characterized using a polyphasic taxonomic approach. The colonies of the isolate were found to be yellow and highly mucoid. Comparative analysis of the 16S rRNA gene sequence showed that this isolate constitutes a distinct phyletic line within the genus Lysobacter, displaying >3 % sequence divergence with respect to recognized Lysobacter species. The generic assignment was confirmed by chemotaxonomic data, which revealed the presence of a fatty acid profile characteristic of members of the genus Lysobacter and consisting of saturated, unsaturated, straight-chain and branched-chain fatty acids as well as iso-C 11 : 0 3-OH as hydroxylated fatty acid, and the presence of an ubiquinone with eight isoprene units (Q-8) as the predominant respiratory quinone. The genotypic and phenotypic data show that strain IMMIB APB-9T merits classification as representing a novel species of the genus Lysobacter, for which the name Lysobacter defluvii sp. nov. is proposed. The type strain is IMMIB APB-9 T (=CCUG 53152 T =DSM 18482 T ).
We describe a new genus of mesophilic actinomycetes, for which we propose the name Lentzea. The strains of this genus form abundant aerial hyphae that fragment into rod-shaped elements. Whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar (wall chemotype 111). The phospholipid pattern type is type PI1 (phosphatidylethanolamine is the characteristic phospholipid); the major menaquinone is MK-9. The fatty acid profile comprises saturated, unsaturated, and branched-chain fatty acids of the is0 and anteiso types in addition to tuberculostearic acid (lOMe-C,,:,). A 16s ribosomal DNA sequence analysis revealed that the genus Lentzea is phylogenically related to the genera Actinosynnema, Sacchurothrix, and Kutzneria. The type species of this genus is Lentzea albidocupillatu sp. nov.; the type strain of this species is strain IMMIB D-958 (= DSM 44073).
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