Wheat grass, one of the members of Poaceae family, has been considered for very efficient therapeutic drugs. Current study was aimed at evaluation of antimicrobial properties of wheat grass extracts. The 7 th. , 14 th. , and 21 st. day wheat grass extracts of five different solvents (water, ethanol, methanol, ethyl acetate and hexane) were assayed for antimicrobial activity using turbidity tests. All these extracts showed antibacterial activity against seven food borne pathogens. Amongst them hexane extracts from 7 th day old wheat grass showed maximum antibacterial activity especially more against Yersinia enterocolitica and Listeria monocytogenes. The HPLC purified extract was observed to create pores on the cell wall of the bacterial cells as observed under Scanning Electron Microscope and also influenced flattening and shrinkage of bacterial cells indicating probable effect on the membrane of the pathogenic bacteria.
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