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Evaporation is one of the most important components in the energy and water budgets of lakes and is a primary process of water loss from their surfaces. An artificial neural network (ANN) technique is used in this study to estimate daily evaporation from Lake Vegoritis in northern Greece and is compared with the classical empirical methods of Penman, Priestley-Taylor and the mass transfer method. Estimation of the evaporation over the lake is based on the energy budget method in combination with a mathematical model of water temperature distribution in the lake. Daily datasets of air temperature, relative humidity, wind velocity, sunshine hours and evaporation are used for training and testing of ANN models. Several input combinations and different ANN architectures are tested to detect the most suitable model for predicting lake evaporation. The best structure obtained for the ANN evaporation model is 4-4-1, with root mean square error (RMSE) from 0.69 to 1.35 mm d −1 and correlation coefficient from 0.79 to 0.92.