A technique of repetitive nerve stimulation of the facial nerve with recording from the nasalis and orbicularis oculi muscles was developed and tested in 15 healthy subjects. Quality criteria were that the stimulus artifact should return to baseline before the onset of the compound muscle action potential (CMAP), and that the CMAP should begin with a negative phase, be biphasic in shape, and have an amplitude of over 1 mV. Repetitive nerve stimulation was only performed if all four criteria were met, which was the case in all 15 subjects for the nasalis muscle, but in only 5 subjects for the orbicularis oculi muscle. The largest observed decrement was 9% in area. Reptitive nerve stimulation of the nasalis muscle is thus feasible, but its diagnostic utility remains to be established in patients with disorders of neuromuscular transmission.