The skeletal remains of 18 individuals interred at the ancient Maya site of Caledonia (100 to 1000 C.E.), located in the Cayo District of Belize, w ere sampled for stable carbon and nitrogen isotope analysis in order to reconstruct their diet. Stable carbon and nitrogen isotope ratios in 18 bone collagen samples and stable carbon isotope ratios in bioapatite from 15 bone and 6 tooth enamel samples were assessed. Small sample sizes precluded the assessment of dietary variation with respect to age, sex, social status or time period among the Caledonia Maya. However, the sampled individuals consumed a varied diet consisting of maize, supplemented with some C 3 plants, terrestrial herbivores and/or lower order freshwater resources such as snails and molluscs and possibly maize-fed animals. This dietary variability with an emphasis on maize is unsurprising given the biological diversity surrounding the site and the known importance of this crop to the ancient Maya. As expected, the isotopic values from Caledonia are similar to those from nearby sites from similar time periods. However, four individuals exhibit a marine dietary signature, possibly indicating inland trade of marine resources from coastal sites, or the migration of coastal people to Caledonia. This study demonstrates the validity of sampling small, fragmented collections from minor Maya centres in order to gain valuable insight into ancient Maya dietary practices.
The analysis of stable sulfur isotopes from human and animal tissues is a relatively recent development in archaeological dietary studies. In human diets, sulfur is ultimately derived from soils at the base of the food chain, which are influenced by local lithology, hydrology, and atmospheric circulation. Plants assimilate local soil δ
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S values into amino acids that are incorporated into the tissues of their consumers, making it possible to differentiate the source of dietary protein (i.e., marine, terrestrial, or freshwater) and identify individuals with nonlocal diets. Recent developments in method and theory have allowed sulfur isotope analysis to be more routinely employed in isotopic studies of archaeological diet, although several key areas require further research. When applied in conjunction with other isotopic assays and interpreted within the environmental and archaeological contexts, stable sulfur isotope analysis offers a powerful and complementary tool for the investigation of archaeological diets.
Maya archaeologists have long been interested in understanding ancient diets because they provide information about broad-scale economic and societal transformations. Though paleodietary studies have primarily relied on stable carbon (δ13C) and nitrogen (δ15N) isotopic analyses of human bone collagen to document the types of food people consumed, stable sulfur (δ34S) isotope analysis can potentially provide valuable data to identify terrestrial, freshwater, or marine/coastal food sources, as well as determine human mobility and migration patterns. Here we assess applications of δ34S for investigating Maya diet and migration through stable isotope analyses of human bone collagen (δ13C, δ15N, and δ34S) from 114 individuals from 12 sites in the Eastern Maya lowlands, temporally spanning from the Late Preclassic (300 BCE—300 CE) through Colonial periods (1520–1800 CE). Results document a diet dominated by maize and other terrestrial resources, consistent with expectations for this inland region. Because δ34S values reflect local geology, our analyses also identified recent migrants to the Eastern lowlands who had non-local δ34S signatures. When combined with other indicators of mobility (e.g., strontium isotopes), sulfur isotopic data provide a powerful tool to investigate movement across a person’s lifespan. This study represents the largest examination of archaeological human δ34S isotope values for the Maya lowlands and provides a foundation for novel insights into both subsistence practices and migration.
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