A new series of chiral 5-substituted bis-oxazolidinones containing an acylsulfonamide moiety has been synthesized starting from chlorosulfonyl isocyanate, (L)-ethyl lactate, and oxazolidin-2-ones. All the reactions were conducted at ambient temperature, and the N-acylsulfonamide bis-oxazolidin-2-ones were obtained with high yields within 2 h. Some of the newly synthesized compounds were evaluated in vitro against the virulent strain RH of Toxoplasma gondii and the human lymphocytes, and showed promising results.
The title compound, C21H21N3O7S, contains an oxazolidinone ring and a sulfonamide group, both characteristic for biologically and pharrmaceutically active compounds. Both stereogenic centres reveal an S absolute configuration. The two oxazolidinone rings are in an envelope conformation with the methylene carbon flap atoms deviating by 0.428 (1) and 0.364 (2) Å from the best least-square planes formed by the four other ring atoms. An intramolecular N—H⋯O hydrogen bond contributes to the folded conformation of the molecule. In the crystal, weak intermolecular C—H⋯O interactions connect the molecules into helices along the the twofold screw axes.
Aim: Our work is devoted to a serological study of avian influenza disease in broiler chickens, after five different vaccine protocols. Methods: a total of 500 broiler chicks (day-old) were equitably divided into 5 lots. in each batch a specific vaccination protocol was followed. The relative level of serum antibodies against H9N2 virus present in the samples is determined at days: 1, 10, 24, 33, and 51 of age. Results: for batches 1 and 2: the kinetics of the antibodies present a stable curve not associated with significant modifications (p> 0.05) during the entire experimental period, whereas for batches 3, 4 and 5: a peak is observed at D 33 for the first two batches and at D51 for the last Conclusion: the development of active immunity conferred by vaccinations has not been proven because in groups 3, 4 and 5 the titers of antibodies reach high values only in the case of response to the provoked infectious condition and not following immunization by vaccines. Keywords: antibodies, ELISA method, immunity, serotype H9N2, vaccine.
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