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As fuzzy c-means clustering (FCM) algorithm is sensitive to noise, local spatial information is often introduced to an objective function to improve the robustness of the FCM algorithm for image segmentation. However, the introduction of local spatial information often leads to a high computational complexity, arising out of an iterative calculation of the distance between pixels within local spatial neighbors and clustering centers. To address this issue, an improved FCM algorithm based on morphological reconstruction and membership filtering (FRFCM) that is significantly faster and more robust than FCM is proposed in this paper. First, the local spatial information of images is incorporated into FRFCM by introducing morphological reconstruction operation to guarantee noise-immunity and image detail-preservation. Second, the modification of membership partition, based on the distance between pixels within local spatial neighbors and clustering centers, is replaced by local membership filtering that depends only on the spatial neighbors of membership partition. Compared with stateof-the-art algorithms, the proposed FRFCM algorithm is simpler and significantly faster, since it is unnecessary to compute the distance between pixels within local spatial neighbors and clustering centers. In addition, it is efficient for noisy image segmentation because membership filtering are able to improve membership partition matrix efficiently. Experiments performed on synthetic and real-world images demonstrate that the proposed algorithm

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A great number of improved fuzzy c-means (FCM) clustering algorithms have been widely used for grayscale and color image segmentation. However, most of them are timeconsuming and unable to provide desired segmentation results for color images due to two reasons. The first one is that the incorporation of local spatial information often causes a high computational complexity due to the repeated distance computation between clustering centers and pixels within a local neighboring window. The other one is that a regular neighboring window usually breaks up the real local spatial structure of images and thus leads to a poor segmentation. In this work, we propose a superpixel-based fast FCM clustering algorithm (SFFCM) that is significantly faster and more robust than stateof-the-art clustering algorithms for color image segmentation. To obtain better local spatial neighborhoods, we firstly define a multiscale morphological gradient reconstruction (MMGR) operation to obtain a superpixel image with accurate contour. In contrast to traditional neighboring window of fixed size and shape, the superpixel image provides better adaptive and irregular local spatial neighborhoods that are helpful for improving color image segmentation. Secondly, based on the obtained superpixel image, the original color image is simplified efficiently and its histogram is computed easily by counting the number of pixels in each region of the superpixel image. Finally, we implement FCM with histogram parameter on the superpixel image to obtain the final segmentation result. Experiments performed on synthetic images and real images demonstrate that the proposed algorithm provides better segmentation results and takes less time than state-of-the-art clustering algorithms for color image segmentation.

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Understanding the pattern of gene expression during erythropoiesis is crucial for a synthesis of erythroid developmental biology. Here, we isolated 4 distinct populations at successive erythropoietindependent stages of erythropoiesis, including the terminal, pyknotic stage. The transcriptome was determined using Affymetrix arrays. First, we demonstrated the importance of using defined cell populations to identify lineage and temporally specific patterns of gene expression. Cells sorted by surface expression profile not only express significantly fewer genes than unsorted cells but also demonstrate significantly greater differences in the expression levels of particular genes between stages than unsorted cells. Second, using standard software, we identified more than 1000 transcripts not previously observed to be differentially expressed during erythroid maturation, 13 of which are highly significantly terminally regulated, including RFXAP and SMARCA4. Third, using matched filtering, we identified 12 transcripts not previously reported to be continuously upregulated in maturing human primary

The problem of the fetal electrocardiogram (FECG) extraction from maternal skin electrode measurements can be modeled from the perspective of blind source separation (BSS). Since no comparison between BSS techniques and other signal processing methods has been made, we compare a BSS procedure based on higher-order statistics and Widrow's multireference adaptive noise cancelling approach. As a best-case scenario for this latter method, optimal Wiener-Hopf solutions are considered. Both procedures are applied to real multichannel ECG recordings obtained from a pregnant woman. The experimental outcomes demonstrate the more robust performance of the blind technique and, in turn, verify the validity of the BSS model in this important biomedical application.