Multiple sclerosis (MS) is an organ specific inflammatory disease that considered the main cause of chronic neurologic disability in young and middle-aged adults with increased prevalence in Saudi Arabia. Public awareness MS is generally poor, and this limited understanding delays the early diagnosis and treatment of patients. This study was community based cross sectional study that conducted in different public areas.A structured questionnaire including questions on knowledge and attitude of MS was administered. A total of 900 questionnaires were distributed and 715 were completed the questionnaire. The mean knowledge score among the study cohort was 8.74 ± 2.7 and the mean attitude score was 2.19± 1.8 with no significant variation regarding age and sex of participants. Learning from surrounding people was the only source of information with significantly high knowledge and attitude score. Also, participants who recorded knowing someone with MS was significantly had higher knowledge and attitude scores. Most respondents in our sample suffered from deficiency of background information about MS and health education. Therefore, more intensified awareness programs among population are necessary to ensure reliable information reach the public in order for early detection and management of this serious disease. Also, further researches are needed to identify causes of inadequate knowledge and attitude of MS among Saudi population. More utilization of Media as an important source of MS is important to improve awareness among the community.