Pathogens utilize a repertoire of effectors to facilitate pathogenesis, but when the host recognizes one of them it causes effector-triggered immunity. The Pseudomonas type III effector AvrRps4 is a bipartite effector that is processed in planta into a functional 133 amino acid N-terminus (AvrRps4-N) and 88 amino acid C-terminus (AvrRps4-C). Previous studies found AvrRps4-C to be sufficient to trigger HR in turnip. In contrast, our recent work found that AvrRps4-N, but not AvrRps4-C, triggered HR in lettuce whereas both were required for resistance induction in Arabidopsis. Here, we initially compared AvrRps4 recognition by turnip and lettuce using transient expression. By serial truncation, we identified the central conserved region consisting of 37 amino acids as essential for AvrRps4-N recognition, whereas the putative type III secretion signal peptide or the C-terminal 13 amino acids were dispensable. Surprisingly, the conserved arginine at position 112 (R112) that is required for full-length AvrRps4 processing is also required for the recognition of AvrRps4-N by lettuce. Mutating R112 to hydrophobic leucine or negatively charged glutamate abolished the HR-inducing capacity of AvrRps4-N, while a positively charged lysine at this position resulted in a slow and weak HR. Together, our results suggest an AvrRps4-N recognition-specific role of R112 in lettuce.
Loss-of-function mutations of the tassel-less1 (tls1) gene in maize, which is the co-ortholog of the Arabidopsis boron (B) importer NIP5;1, leads to the loss of reproductive structures (tassels and ears). The tls1 phenotypes can be rescued by B supplementation in the field and in the greenhouse. As the rescue with B supplementation is variable in the field, we investigated additional abiotic factors, potentially causing this variation in controlled greenhouse conditions. We found that the B-dependent rescue of the tls1 mutant tassel phenotype was enhanced when plants were grown with a mix of high pressure sodium (HPS) and metal halide (MH) lamps. Normal and tls1 plants had a significant increase in transpiration and increased B content in the leaves in the greenhouse with the addition of MH lamps. Our findings imply that B transport to the shoot is enhanced through increased transpiration, which suggests that the xylem transpiration stream provides a significant supply of B in maize.
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