The antidiabetic activity of both leaves and MJ-treated cell cultures of Morus nigra was evaluated after their oral administration to streptozotocin-induced diabetic rats. The antidiabetic activity of extracts from leaves given to streptozotocin (STZ)-diabetic rats for 10 days increased with increasing doses of leaves extract up to 500 mg/kg/day. The administration of 500 mg/kg/day of leaves extract reduced the concentration of glucose from 370 ± 7.31 mg/dl (control) to 154 ± 6.27 mg/dl, and a significant increase in the insulin level from 11.3 ± 0.31 μU/ml (control) to 14.6 ± 0.43 μU/ml was recorded. Cell suspension cultures were established from the young leaves of Morus nigra cultivated on modified MS medium supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA), 0.2 mg/l 6-(furfurylamino)purine (kinetin). The changes in cell weight and flavonoid content were monitored between day zero and 12. The linear increase in fresh weight was found to be parallel to flavonoids production. Cell cultures treated with 100 μM methyl jasmonate for 24 hours showed a noticeable increase in level of flavonoids and significant and more effective hypoglycemic activity than that for extract from leaves. The major flavonoids were isolated by TLC and HPLC and identified as rutin, quercetin, Morusin and cyclomorusin by co-chromatography and mass spectrometry in comparison to samples of authentic reference compounds.
Background and aim of the study: diabetes was proposed to be an inflammatory disease. Growing evidence has pointed to a correlation between various proinflammatory cytokines, insulin resistance (IR) and type 2 diabetes (T2DM). Materials and Methods: This study was carried out on one hundred Albino rats, distributed into four groups. Group I: control group, Group II: diabetic rats with no treatment, Group III: diabetic rats treated with Glipenclamid and Group IV: diabetic rats treated with Diclofenac sodium. Blood samples were taken and the following biochemical parameters were done: fasting blood glucose (FBG), serum insulin, aspartate transaminase (AST), Alanine transaminase (ALT), serum Alkaline Phosphatase (ALP), serum protein, serum albumin, serum triglyceride (TGs), serum cholesterol level (TC), High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), Tumor Necrosis Factor (TNF-α) and C-Reactive Protein (CRP). HOMA IR and HOMA B were calculated. Liver samples from all rats were obtained and stained with Hematoxylin and Eosin, Masson's trichrome and Periodic acid-Schiff (PAS) for histological examination. Results: Declofenac caused significant lowering in FBG, lipid profile, TNF-α level, CRP, increased insulin secretion with improved IR and beta cell function compared to the diabetic group. There was a positive correlation between HOMA-IR and CRP; HOMA-IR and serum TNF-α. Liver of diabetic rats showed periportal fibrosis, vacuolated cytoplasm and nuclei and glycogen deposition. These changes improved markedly in Glibenclamide treated groups while liver of Declofenac treated group revealed parenchymal cell necrosis, sinusoidal dilatation with some pyknotic nuclei and marked glycogen deposition. Conclusions: inflammatory pathways may play an important part in IR of T2DM. Therefore, antinflammatory drugs may have a role in diabetes therapy through improving IR because of its insulinsensitizing and anti-inflammatory properties.
Background and aim of the study-Diabetes is a chronic metabolic disease which affects large number of population all over the world. More than 400 traditional medicinal plants have been recorded for helping in controlling such disease. This study investigated effects of some plants used in Saudi Arabia and some other Arab countries as antidiabetic agents. Materials and Methods: One hundred fifty adult male Albino rats were divided into six experimental groups each consist of twenty five rats. The first group was considered as a control group. The rest of groups were affected by induction of experimental diabetes by subcutaneous injection of Alloxan. The second group consisted of diabetic rats without any treatment. The third group was treated by the aqueous extract of mixture contains Foenugreek, Nigella and Termis seeds. The fourth group was treated with the aqueous extract of Nigella sativa seeds, while the fifth group was treated with the aqueous extract of Foenugreek seeds.The sixth one was treated with the aqueous extract of Termis seeds with the administered dose of the plant extracts (100 mg/kg body weight).After four weeks of treatment, different biochemical parameters were performed including estimation of blood sugar level and serum insulin level. Pancreatic and liver samples were obtained and processed for microscopic and quantitative evaluation after staining the prepared sections with both heamatoxylin and eosin as well as a special stain for demonstration of the different pancreatic cells in the Islet of Langerhans. Results: The usage of the mixture or each plant alone corrected the glucose level and insulin level. Microscopically there was definite decrease in the number and diameter of beta pancreatic cells in the diabetic group, while the other pancreatic cells were not affected (alpha and delta cells). The use of medicinal plants in the different groups of this study greatly improved such cellular changes and the level of blood sugar level was corrected. The present results showed that the activity of the mixture was the best when compared with Nigella, Foenugreek and Termis seeds. Conclusions: The water extract of the mixture is the most powerful in amelioration hyperglycemia and most of all damage effects of Alloxan on the liver and texture, hematological parameters, and lipid profile. So it is advised to use the plant mixture as an antidiabetic agent rather than the use of each plant separately. Repeating such study with the use of variable doses may be helpful in better evaluation for the required doses.
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