The present study was designed to throw the light on isolation of Escherichia coli (E. coli) strains accompanied by Infectious bronchitis virus (IBV). Classification and serotyping of bacterial isolates of E.coli were done followed by their antimicrobial susceptibility testing, also isolation and identification of IBV was done. For that purpose, a total of 200 organs, swap and samples were aseptically collected from broilers and layers flocks suffered from respiratory symptoms and general illness from both private and governmental farms during the period from 2015 till the end of 2017. Results revealed that the prevalence of E. coli was 67.5% and IBV was 61.5% in the collected samples. The serological identification showed that the most predominant serotype for E. coli was O 158 and there was a high level of resistance of isolated strains against doxycycline and tetracycline.