Preservation of milk is important as it relates to Capra aegagrus hircus (Alpine goat) milk DNA extraction. We examined the difference in concentration and quality of DNA resulting from the use of a preservation tablet (BronopolTM) versus a preservation solution by Norgen Biotek. When examining the concentration and quality of DNA in goat milk for studies using somatic cells from goat milk, it is ideal to use a substance that has a long-term preservation potential. The concentrations and quality of DNA obtained from goat milk was compared. Two separate trial samples of Alpine goat milk were obtained. The preservation tablet commonly known as B-14 or Bronopol™ was dissolved into one sample of milk. Another sample of goat milk without a tablet used a preservation solution from a Norgen Biotek. All DNA extraction methods followed the Norgen Biotek Corp. manufacturer's protocol. DNA quantity and quality was analyzed using a Thermo Scientific NanodropLite spectrophotometer. The study showed that the traditional Bronopol™ was a better method of preserving and maintaining the integrity of DNA in the somatic cells that are present in Alpine goat milk. This is based on the results obtained following determination of quantity and A260/A280 readings for quality assessment. Thus, the use of Bronopol™ is the preferred method of preserving goat milk for DNA extraction.
Replicate milk samples were obtained from a herd of Alpine Goats on the research farm at Langston University in Langston, Oklahoma. Subsequent DNA extraction and quantitative real time PCR (qRTPCR) were conducted using Norgen Biotek Milk DNA Preservation and Isolation kit followed by amplification with an iQ™ SYBR Green Supermix. Quantitative real time PCR assays detecting Listeria monocytogenes, Staphylococcus aureus, Samonella enterica, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis were conducted. These bacteria are all known to cause Mastitis. Mastitis is an inflammation of the udder that causes chemical changes in the composition of, and subsequent lowering of quality in the milk that is produced by affected goats, further causing difficulty affecting an entire dairy herd. This leads to huge economic losses for producers and risk to consumers. Early detection of pathogens that cause Mastitis should be included in routine diagnostic testing. This study shows a diagnostic procedure that can be utilized for the assessment of safe dairy products from Alpine goats. Presented here are the results of a healthy herd raised at the American Institute for Goat Research.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.