N. 1982, Changes in the level of endogeoous cytokinin-Iike substances in Acer pseudoplatanus embryos during stratification and gennination. -Physiol. Plant. 54: 318-322.The bioassay used to detect and quantify cytokinin activity was ihe Amaranthus test. Free cytokinin-like substatices in embryos ol Acer pseudoplatanus L. fruits increased duritig the first 20 d of fruit stratification at 5°C, but subsequently fell rapidly to values well below the amounts present in the embryos of freshly harvested fruits. These lower levels persisted throughout the remainder of a 60 d stratification period. Bound cytokinins fell during stratification from the highest detected levels present in freshly harvested material to values which were lower by about one third. No peaks of bound cytokinin activity were detected at any stage during stratification. In embryos from fruits stored at 17°C and unable to germinate, both free and bound cytokinins remained at a very low level throughout the 60 d period. Embryos from fruits previously stratified for 60' d showed increases in both free and bound cytokinins during the first 24 h of their incubation at 20°C in light, but after longer incubation periods up to 72 h, cytokinin concentrations decreased again to levels similar to those present at the commencement of the incubation period. Determinations conducted in 1979 and 1980 showed quantitative differences, but similar qualitative changes were observed in the two years. Most of the cytokinin activity was associated with compound{s) that co-chromatographed with zeatin and zeatin riboside.Additional key-words -Amaranthus test.
A. Julin-Tegelman {reprint requests),
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.