Dental caries is caused by acids released from bacterial biofilms. However, the in vivo formation of initial biofilms in relation to caries remains largely unexplored. The aim of this study was to compare the oral microbiome during the initial phase of bacterial colonization for individuals with (CC) and without (NC) cavitated dentin caries lesions. Bovine enamel slabs on acrylic splints were worn by the volunteers (CC: 14, NC: 13) for in situ biofilm formation (2 h, 4 h, 8 h, 1 ml saliva as reference). Sequencing of the V1/V2 regions of the 16S rRNA gene was performed (MiSeq). The relative abundances of individual operational taxonomic units (OTUs) were compared between samples from the CC group and the NC group. Random forests models were furthermore trained to separate the groups. While the overall heterogeneity did not differ substantially between CC and NC individuals, several individual OTUs were found to have significantly different relative abundances. For the 8 h samples, most of the significant OTUs showed higher relative abundances in the CC group, while the majority of significant OTUs in the saliva samples were more abundant in the NC group. Furthermore, using OTU signatures enabled a separation between both groups, with area-under-the-curve (AUC) values of ~0.8. In summary, the results suggest that initial oral biofilms provide the potential to differentiate between CC and NC individuals.

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This paper examines the administrative and legal structure of the wastewater sector in Turkey, identifying its shortcomings and their causes, and suggests solutions. The study begins by outlining the existing administrative and legal structure, then focusing on the areas where difficulties are faced and seeking solutions to them. Those administrative and legal facets which have produced improvements and/or function effectively have been analysed so as to provide examples for other areas. When examining the sector it was observed that problems common to every area are the failure to adequately define powers and responsibilities, the lack of coordination between sectoral organisations, and the fact that attention is generally concentrated on routine matters of secondary importance. The fact that numerous organisations share powers in the same area causes confusion. Plurality also creates difficulties where enforcement is concerned. The existence of more than one set of regulations relating to the same area gives rise to loopholes. At the same time, unrealistic, and hence unenforceable standards undermine the effectiveness of sanctions. It has been concluded that a single organisation should be invested with the legal powers to lay down the principles of water management, that environmental courts should be set up to enforce legislation by means of sanctions; and that for this purpose existing legislation should be reexamined and revised as necessary and/or brought up to date.

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