The use of bacteria in improving the quality of salt on a laboratory scale is still minimal. Bacteria are found in seawater as raw materials for various types of salt. One of the bacteria that are tolerant to salinity levels in raw saltwater is halophilic bacteria. Exploration of halophilic bacteria isolates contained in seawater as raw material for salt is an effort to provide initial information on the use of these bacteria in improving the quality and quality of salt. This study aims to determine the morphological characteristics and gram grouping of halophilic bacteria contained in raw water, reservoir water, and evaporator water during the traditional salt production process. The methods used in this study were bacterial isolation, purification, and gram staining test. Morphological characteristics were carried out by visual observation of bacterial colonies formed in Petri dishes, while the gram test of bacteria was carried out by staining pure isolates. Morphological characteristics and groupings of gram bacteria were observed under a CX43RF binocular microscope with a digital camera type MDCE-5C. The results of this study found 2 isolates circular in raw saltwater, 5 isolates in irregular, filamentous and circular shapes in reservoir water, and 3 isolates in circular and filamentous shape in purification water. The bacterial isolates found varied in the form of groups of gram-negative bacteria and groups of positive bacteria, while the predominant form of bacteria was bacilli. The results of this study are expected to be initial information that can be used as a reference to improve the quality and quantity of salt production.
Kualitas air laut seperti mineral dan cemaran sangat berpengaruh terhadap hasil produksi garam. Salah satu indikator penurunan kualitas air laut adalah fosfat. Tujuan dari penelitian ini adalah untuk mengetahui hubungan fosfat terhadap kualitas bahan baku garam di Desa Padelegan. Penelitian ini menggunakan metode Purposive Sampling berdasarkan zonasi perairan, dimana sampel air diambil dan di analisa merujuk pada SNI 06-6989.31-2005. Kandungan fosfat tertinggi diketahui berada pada muara sungai dan pantai sebesar 0.1686745 mg/L dan terendah di badan sungai sebesar. 0.12851 mg/L. Hal ini dapat diakibatkan karena daerah pantai dan muara sungai merupakan daerah yang berdekatan dengan sumber cemaran fosfat. Berdasarkan PP RI No.22 Tahun 2021 tentang baku mutu air konsumsi kandungan fosfat di perairan Desa Padelegan masih di bawah ambang batas dan melebihi ambang batas untuk kehidupan biota. . Kandungan fosfat yang tinggi berpengaruh terhadap kondisi fisik garam seperti warna yang tidak putih, serta memberikan cita rasa yang menyimpang (pahit) setelah proses produksi.
Madura memberikan kontribusi terbesar terhadap produksi garam di Jawa Timur. Produksi garam menghasilkan limbah yang disebut bittern. Bittern merupakan air sisa kristalisasi garam yang berbentuk cairan dengan kadar kepekatan >29o Be dan memiliki kandungan utama berupa ion magnesium. Selama ini, hasil bittern dari tambak garam rakyat Kabupaten Pamekasan, Madura digunakan kembali untuk proses produksi garam, dimana kegiatan tersebut dapat menurunkan kualitas garam. Dalam skala industri, bittern dapat digunakan sebagai bahan baku magnesium, namun diperlukan suatu metode untuk memisahkan senyawa lainnya agar tidak mengganggu proses ekstraksi magnesium. Ion sulfat (SO₄²ˉ) merupakan ion terbanyak kedua yang terkandung dalam bittern, sehingga pemisahan ion sulfat merupakan suatu strategi untuk meningkatkan kualitas bittern sebagai bahan baku magnesium klorida. Pada penelitian ini, proses pemisahan ion sulfat menggunakan reagen kalsium klorida dihidrat (CaCl₂.2H₂O) yang ditambahkan pada bittern dengan perbandingan antara SO₄²ˉ dan CaCl₂.2H₂O yaitu P1 = 1:0,90 ; P2= 1:0,95; P3 1:1; P4=1:1,05; dan P5= 1:1,1. Berdasarkan hasil penelitian, dapat disimpulkan bahwa kandungan sulfat pada bittern yaitu 41257,14±757,14 mg/L. Kadar sulfat pada filtrat dengan perlakuan P₁ = 4,14±0,43 mg/L; P₂ = 5,38±0,22 mg/L; P₃ = 7,57±1,14 mg/L; P₄ = 6,57±0,57 mg/L; dan P₅ = 7,48±0,46 mg/L. Penambahan CaCl₂.2H₂O berpengaruh terhadap kadar sulfat pada bittern yang dapat dilihat dari hasil uji ANOVA. Perlakuan optimal dari uji lanjut DNMR yaitu pada perlakuan ratio molar 1:0,9 dengan nilai rata-rata 4,1429 mg/L dimana dilihat dari rata-rata paling kecil dari perlakuan lainnya. Hasil penelitian ini menjadi penelitian dasar untuk melakukan optimasi ekstrak magnesium klorida dari bahan baku bittern. Madura provides the largest contribution to salt production in East Java. Salt production generates a waste called bittern. Bittern is the residual liquid of salt crystallization with a concentration of 29oBe and has the main content of magnesium ions. Unfortunately, bittern from the salt pond in Pamekasan Regency, Madura is reused for the salt production process, whereby this activity can reduce the quality of salt. On an industrial scale, bittern can be used as a raw material for magnesium, but a method is needed to separate other compounds so it does not interfere with the magnesium extraction process. Sulfate ion (SO₄²ˉ) is the second most abundant ion contained in bittern. Thus, the separation of sulfate ions is a strategy to improve the quality of bittern as a raw material for magnesium chloride. In this study, the separation process of sulfate ions using calcium chloride dihydrate reagent (CaCl₂.2H₂O) added to bittern with a ratio between SO₄²ˉ and CaCl₂.2H₂O, namely P1 = 1:0,90; P2= 1:0,95; P3 1:1; P4=1:1,05; dan P5= 1:1,1. Based on the research results, it can be concluded that the sulfate content in bittern is 41257.14 ± 757.14mg/L. Sulfate levels in the filtrate were P₁ = 4.14±0.43 mg/L; P₂ = 5.38±0.22 mg/L; P₃ = 7.57±1.14mg/L; P₄ = 6.57±0.57mg/L; and P₅ = 7.48±0.46 mg/L.The addition of CaCl₂.2H₂O has an effect on the sulfate content of bittern which can be exhibited from the ANOVA test results. The optimal treatment of the DNMR test is the treatment of the molar ratio of 1: 0.9 with an average value of 4.1429 mg/L, which is referred from the smallest average of other treatments. The results of this study serve as basic research to optimize the extract of magnesium chloride from bittern as raw material.
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