A novel microencapsulation technique for Lactobacillus casei 431 cells was developed, and the bacterial stability was studied. Cryoprotective solutes were incorporated in the encapsulation mix to improve freeze-drying survival. The losses of viable cells during drying were recorded as 1.7, 0.84 and 0.38 log Colony-forming units (cfu)/g for control and samples with trehalose and lactose, respectively. During the simulated gastric-intestinal transit, trehalose and lactose contributed to higher survivals of 3.1 and 3.0 log cfu/g, respectively, in gastric fluid and 1.3 log and 0.9 log cfu/g, respectively, in 1% bile extract solution. Higher temperature storage was found to be detrimental for cell viability.
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