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Deinococcus radiodurans R1 is a highly radio-tolerant bacterium. Depending on the nutrient availability D. radiodurans R1 exists in three morphologies viz. monococcal, diplococcal and tetracoccal. In this study, we examined whether nutrition-induced morphotypes of D. radiodurans showed similar DNA damage upon gamma radiation exposure. Total DNA damage after radiation exposure was estimated by comparing percent double-strand breaks (DSBs) in genomic DNA. It was found that all three morphotypes exhibited different radiation tolerances which were also dependent on the radiation dose given. Monococcal forms were found to be most radio-tolerant at most of the tested radiation doses. Results showed that these nutrient-starved-condition induced morphotypes show lesser DNA DSBs upon irradiation, hence show higher radio-tolerance.

A precise quantification of Co(II)EDTA complex is required to develop bioremediation approaches for Co(III)EDTA complex that is generated in various industrial processes. It is cumbersome to quantify Co(II)EDTA in a mixture of Co(II)EDTAand Co(III)EDTAby normal UV-visible spectrophotometric methods as both the complexes absorb significantly at 490 nm, which is the λ max of Co(II)EDTA. Whereas other sophisticated techniques such as gas chromatography, atomic absorption spectrophotometer, inductively coupled plasma-atomic emission spectroscopy can measure individual species of Co(II)EDTA or Co(III)EDTA when present alone but are ineffective to measure the Co(II)EDTAprecisely when it is mixed with Co(III)EDTA. Hence, an attempt has been made to develop a spectrophotometric method for its quantification. This paper describes the development of a simple and economic dual wavelength spectrophotometric method for the determination of Co(II)EDTAin a mixture of Co(II)EDTA and Co(III)EDTA complexes. The wavelength pair, 490-580 nm was optimized for the measurement of absorbance for this dual wavelength method of determination of Co(II)EDTA in the presence of Co(III)EDTA. Int J Appl Sci Biotechnol, Vol 3(4): 584-587

This study shows that phototrophic granules are more efficient as compared to microbial granules or monoculture bacterial culture and are a self-sustainable system to be used in bioremediation process of environmental contaminants.