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his study deals with the antibacterial activity of Sea buckthorn (SB) (Hippophae rhamnoides L.) grown in the Republic of Moldova. Eight sea buckthorn species were investigated: R1, R2, R4, R5, C6, AGG, AGA, Pomona, 2020 harvest, from Dubasari district, Pohrebea village of the Republic of Moldova. The sea buckthorn fruit was harvested during the complete sweeping phase. The antibacterial efficacy of Sea buckthorn on different microbial cultures (Staphylococcus aureus, Bacillus subtilis, Salmonella Typhimurium, Escherichia coli, Candida albicans) causing infections/diseases was investigated by agar disc diffusion method. The inhibition zones ranged from 12 to 30 mm: Staphylococcus aureus ATCC 25923 (21-30 mm); Bacillus subtilis ATCC6633 (19-29 mm); Salmonella Typhimurium ATCC 14028 (13-18 mm); Escherichia coli ATCC 25922 (12-18 mm). In the case of Candida albicans ATCC 10231, this fungal pathogen was resistant to SB. It was found that antimicrobial efficacy of SB depends on the species, concentration, and form of use (fruit puree, extracts with different solvents and powder). Results suggested that SB might be a valuable ingredient for the development of safe products for consumption.

The problem of functional foods with bioactive components of natural origin is current for the food industry. Plant extracts rich in polyphenols with antioxidant and antimicrobial activity are a promising source for use in improving the quality and characteristics of fresh meat and meat products. In this context, the purpose of the present study was to evaluate the physico-chemical, microbiological, sensory properties of sausages prepared with the addition of lyophilized extract of basil, thyme or tarragon. For the beginning, the total amount of polyphenols, the antioxidant and antimicrobial activity of the extracts obtained from three spices were evaluated. In the sausages previously infected with Staphylococcus aureus and Escherichia coli it was observed that there is a much larger number of colonies of microorganisms in the control sample compared to the other samples within 24 and 48 h. Moreover, following the addition of sausage extracts, no changes were found regarding their sensory acceptability.

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Milk is an excellent source of well-balanced nutrients and shows, also, biological activity that influence digestion, metabolic responses to absorbed nutrients and disease resistance. In this paper, 3 yogurt samples were made from a mixture of goat’s and cow’s milk: 3: 1; 1: 1; 1: 3. The quality characteristics were evaluated by means of physico-chemical indices (acidity, pH, syneresis, viscosity, total dry matter, fat content, protein content, aw, ash content), microbiological (total number of microorganisms and yeasts) and organoleptics. For the yogurt made from milk mixture in a ratio of 1: 1, higher quality indices were observed than the other samples, namely: an increase in the number of lactic acid bacteria more intense (0.98 ± 0.024 μ), respectively a higher lactic acid content (63,398 ± 0.022 g · dm-3), lowering of the pH value (6.75 ± 0.03-4.55 ± 0.01) and more special organoleptic characteristics: fine and homogeneous curd, without gas bubbles, pleasant taste and smell, without syneresis. This yogurt sample also showed the best results during the storage period (1-15 days).

The yogurt was obtained from a combination of 50% goat's milk and 50% cow's milk with the inclusion of scald fruits of aronia (Aronia melanocarpa), raspberries (Rubus idaeus), strawberry (Fragaria xanassa). Physico-chemical and microbiological indices were determined, according to standard methods, after manufacture and storage, after 1, 5, 10, 15 days. Compared to other samples, yogurt with aronia showed the best values of the dynamics specific to the development of microorganisms: 2.93.107 cfu/ml; the growth rate of lactic acid bacteria at fermentation 0.95 μ; physico-chemical indices: titratable acidity 85 ± 0.078⁰T, pH 4.28 ± 0.002, water activity 0.875 ± 0.025; total dry matter 18.45 ± 0.31%, viscosity 2500 ± 0.023 mPa s, ash content 0.89 ± 0.10% and the optical density 2.531 ± 0.054 nm. Yeasts and molds were not detected in any of the samples. From a physico-chemical point of view, in storage, in all fruit yogurt samples the titratable acidity showed increasing values, pH remaining in the range of permissible values. In storage fruits formed an association to control the microbiological risk and stability of yogurt. Fruit yogurt shows a synergism with Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis subsp lactis biovar diacetilactis. The overall Pearson coefficient (Pc = f(pH and MC) for all fruit yogurt samples is -0.95066.

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