Introduction: The purpose of the study is to compare the effects of genistein, estradiol, estradiol/progesterone combination on the bone mineral density and biomechanical properties of ovariectomized rats’ bone.
Methods: 50 female adult Sprague-Dawley rats were divided into five groups. Bilaterally ovaeriectomy were performed in all groups except the sham-operated group. Groups were a sham-operated group and a control group (water was given), estradiol treated group (17-β estradiol 0.015 mg/kg per day), genistein treated group (genistein 10 mg/kg per day), and an estradiol/progesterone combination group (17-β estradiol 0.015 mg/kg plus drosperinone 0.028 mg/kg per day). The water or hormones were implemented in relevant groups for eight weeks by orogasthric catheter. The bone mineral density and biomechanical properties of the femur were analyzed.
Results: Genistein, estradiol, and estradiol/progesterone groups increased bone mineral density significantly compared to the control group. In diaphysis and metaphysis bending test, all groups had higher peak load values than the control group. There were statistically significant differences between the estrogen/progesterone group and control group in diaphysis bending with regard to peak load. There were statistically significant differences between the estradiol and control groups in metaphysis bending with regard to peak load. In axial rotation test, all groups had higher peak torque values than the control groups.
Conclusions: Genistein, estradiol and estrogen/progesterone combination improved the biomechanical properties of the ovariectomized rat bone. Genistein which has less side effects may be considered as an alternative in the treatment of postmenopausal osteoporosis.
The pullout performance of various pedicle screws after artificial fusion process was investigated in this study. Normal, cannulated (cemented), novel expandable and normal (cemented) pedicle screws were tested. Polyurethane foams (Grade 10 and Grade 40) produced by casting method were used as test materials. The instrumentation of pedicle screws has been carried out with production of foams, simultaneously. For cemented pedicle screws, 3D models were prepared with respect to the anteriosuperior and oblique radiographs by using PMMA before casting procedure. Pullout tests were performed in an Instron 3369 testing device. Load versus displacement graph was recorded and the ultimate force was defined as the pullout strength sustained before failure of screw. As expected, the pullout strengths of pedicle screws in postfusion are higher than before fusion. Pullout strengths increased significantly by artificial fusion in Grade 10 foams compared to Grade 40 foams. Additionally, while the pullout strengths of normal, cannulated and novel expandable pedicle screws increased by artificial fusion, cemented normal pedicle screws had lower pullout values than before fusion in Grade 40 foams. When the cemented normal pedicle screws are excluded, other screws have almost similar pullout strength level. On the other hand, the pedicle screws have different increasing behaviour also, there is no correlation between each other. As a result, the novel expandable pedicle screws can be used instead of normal and cannulated ones due to their performances in non-cemented usage.
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