Angiogenesis is one of the most important processes for normal lung development. Oxidative stress can impair the pulmonary angiogenesis, leading to chronic lung disease or Bronchopulmonary dysplasia (BPD).ObjectiveTo investigate the protective effects of EC-SOD overexpression on pulmonary angiogenesis on neonates following exposure to acute hyperoxia.Design/MethodsTransgenic (TG) and wild-type (WT) neonatal mice (10 mice per group) were exposed either to air (control group) or 95% O2 for 7 days starting at birth. After exposure, all animals were sacrificed. ROS concentration was measured in lung homogenates using OxiSelect ROS assay kit. Mean vascular density (MVD) was measured using anti CD34 staining. RNA was extracted and the angiogenesis markers, VEGF, VEGFR1 and VEGFR2 and PECAM-1 were analyzed by RT-q PCR. VGEF protein was measured using Western blots. Endothelial progenitor cells (EPCs) was assayed by flow cytometer.ResultsThere was a significant reduction of ROS in TG hyperoxic neonate group (156±14.2) compared to WT hyperoxic animals (255±35.1). Evaluation of MVD, using anti-CD34, showed marked significant increase of MVD in the TG group following hyperoxic exposure (85±12) in comparison to the WT hyperoxic group (62±8.4), (P<0.05). Among the hyperoxic groups, both RNA and protein of VEGF expression were significantly reduced in the WT animals compared to the TG group (P<0.05). The same trend was found in VEGFR 1 and 2 which were significantly reduced in WT group compared to the TG group (P<0.05). There was no significant difference between hyperoxia TG and control group (P>0.05). PECAM expression was significantly reduced in both hyperoxic compared to normoxic groups (P<0.05). EPC’s showed significant reduction in WT hyperoxic group compared to others (P>0.05).ConclusionsEC-SOD plays a key role in preserving angiogenesis by scavenging free radicals which has an inhibitory effect on angiogenesis process in neonatal mice lung following exposure to hyperoxia.
Indoor human detection and localization sensors are at the base of many automation and monitoring systems. This work presents an indoor tagless passive human body identification method. It uses a load-mode capacitive sensor to detect the differences in the conductive and dielectric properties of the human body due to differences in body constituency. The experimental results show that four male individuals with similar height but different body mass index (BMI) standing at 70 cm in front of a chest-level 16 cm x 16 cm sensor plate determine different capacitance-frequency characteristics over a 5 kHz-160 kHz range, which can be used to identify the person.
Although useful for many applications, the practical use of tagless remote human identification is often hampered by privacy, usability, reliability or cost concerns. In this article, we explore the use of capacitive sensors, which appear to address most of these concerns, to identify different persons based on the unique electric and dielectric properties of their bodies given by their specific tissue composition. We present experimental results obtained by measuring the capacitance of a 16 cm×16 cm transducer plate 70 cm in front of different human bodies at different frequencies in the 5 kHz-160 kHz range. The measurements show clearly distinct signatures of capacitance variation with frequency for each person in the experiment, even after accounting for capacitance variations due to different body mass or physical dimensions. This work focuses on the contactless identification of human body using capacitive sensors in smart home environments.
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