This study aims to determine the levels of bioethanol from (black sticky rice, white sticky rice and cassava rice) with variations in yeast mass as much as 5 grams, 10 grams and 15 grams knowing the optimum time variation and knowing ethanol levels according to SNI. This research was carried out by hydrolysis or boiling of ingredients (black sticky rice, white sticky rice and cassava rice) using distilled water for 30 minutes, then fermentation using yeast saccharomyces cereviseae and distillation process using a complete destilator to obtain ethanol from fermented then tested with a pen refractometer to determine whether or not ethanol is formed from the distillation process. The selected sample was then tested for ethanol content using a gas chromatography tool. The results of this study are known by testing gas chromatography to find out the ethanol content contained in the distilled sample, the results of ethanol, black glutinous rice with 96 hours fermentation time 92.29% ethanol, and white glutinous rice with fermentation time 96 hours of 100% ethanol and cassava with 96 hours of fermentation time of ethanol content of 98.10%. Keywords: Black Sticky Rice, White Sticky Rice, Cassava, Fermentation, Ethanol
Krisis energi sudah dirasakan oleh beberapa negara, kelangkaan bahan bakar harus segera diatasi. Banyak negara-negara di eropa sudah mulai menggunakan bahan bakar alternatif yang berasal dari tumbuh-tumbuhan. Beras ketan dan singkong dapat diubah menjadi bioetanol dengan metode fermentasi. Metode yang digunakan dalam penelitian ini adalah eksperimental dengan cara fermentasi kemudian melakukan destilasi untuk memperoleh bioetanol. Hasil bioetanol kemudian dideteksi untuk mencari optimasi waktu fermentasi yang tepat, pengujian dengan Pen refractometer dan Gas Chromatography. Hasil penelitian penambahan massa ragi dari 5-15 gram terbentuk kadar etanol yang semakin meningkatan dari 3,5-18,5 %, hal ini menunjukkan penambahan jumlah ragi berpengaruh signifikan terhadap hasil fermentasi bioetanol. Pengaruh ketidakstabilan mikroba yang mengurai karbohidrat menjadi etanol, saat fermentasi mengakibatkan terbentuknya senyawa-senyawa asam. Hal ini yang menyebabkan penambahan massa ragi tidak signifikan terhadap hasil fermentasinya. Waktu fermentasi yang optimal terjadi pada hari ke-4 dengan jumlah waktu 96 jam. Hasil Uji Gas kromatografi dua bahan memenuhi syarat SNI yaitu: Hasil fermentasi Ketan Putih dan Singkong.
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