Introduction Metalloproteinases-3 (MMP3) are the main enzymes involved in cartilage degradation. Several genetic and non-genetic factors can increase the expression of MMP3 in patients with osteoarthritis (OA). This study aims to analyze the risk factors associated with the expression of the MMP3 gene rs679620 fluid synovial knee OA patients. Methods A cross-sectional study was conducted at the orthopedic polyclinic Arifin Achmad Riau Province and Ibn Sina Hospital in Pekanbaru City. Ninety women who experienced knee OA were taken as samples by consecutive sampling and then signed the informed consent. Data were obtained through interviews using a questionnaire about characteristics, followed by weight and height measurements. Interleukin-1 β (IL-1β) and Tumor Necrosis Factor (TNF-α) were examined from the synovial fluid using the enzyme-linked immunosorbent assay (ELISA) method. The Metalloproteinases-3 (MMP3) gene polymorphism rs679620 was obtained from the DNA analysis of joint fluid results in the Biomedical Laboratory of the Faculty of Medicine, Andalas University. The data was processed computerized and then analyzed using the correlation Spearman-Rank, and chi-square tests. The results of statistical analysis are considered significant if the p-value is 0.05. Results The MMP3 rs679620 gene polymorphism of the mutant type was 88.9%, with the same proportion of AG and GG alleles (44.4%). Subjects aged ≥ 60 years were 53.3%, 85.6% did not work and 84.4% had menopause. The highest degree of OA was grade 2 (53.3%), most of whom had a risky nutritional status (84.4%). The median expression of the MMP3 rs679620 gene was 5.28 copies number. There is a significant relationship between MMP3 gene polymorphism rs679620, age, IL-1β, and TNF-α with MMP3 gene expression rs679620. There is no significant relationship between BMI, work status, and menopausal status with MMP3 gene expression rs679620. Conclusion. MMP3 gene polymorphism rs679620, age, levels of IL-1β and TNF-α are risk factors for increased MMP3 gene rs679620 expression in female knee OA.

Background: IL-1β and TNF-α are vital inflammatory cytokines in the pathophysiological process of Osteoarthritis (OA). Several risk factors can increase the expression of these cytokines, such as BMI, physical activity, and menopausal status.   Aims: This study aims to determine the relationship of modifiable factors with synovial fluid IL-1β and TNF-α levels in knee OA women.   Methods: The cross-sectional study was conducted at the orthopedic poly hospital Arifin Achmad Riau Province and Ibnu Sina Hospital in Pekanbaru City. A total of 93 women with knee OA were taken as samples by consecutive sampling. Data were obtained directly from respondents by conducting interviews using a questionnaire, measuring weight and height, examining levels of IL-1β and TNF-α from the synovial fluid using the enzyme-linked immunosorbent assay (ELISA) method. The data were processed computerized using the Person correlation test, One Way Anova, and t-Test. The statistical analysis results were considered significant if the p-value was 0<05.   Results: the average age of subjects was 60.67 + 9.99 years, 87.8% aged > 40 years, 84.9% had menopause, and at most had moderate physical activity degrees (51.6%). The mean BMI was 27.18+4.17, the average of IL-1β 424.73+188.01 pg/mL, and TNF-α 105,17+48.98 ng/L. There was a significant positive correlation with moderate strength between BMI and levels of IL-1β and TNF-α synovial fluid (p=0.037, r=0.217, and p=0.047, r=0.207).   Conclusion: BMI is a risk factor for IL-1β and TNF-α levels in synovial fluid of knee joints in women with OA, but physical activity and menopausal status are not risk factors.