Objetivo: Evaluar el método de acondicionamiento y desinfección de Sagittaria macrophylla zucc. (Alismataceae) de la Cienega de Lerma, para su propagación y conservación. Diseño/ metodología/ aproximación: En el invernadero se estableció un diseño completamente al azar con tres repeticiones para evaluar dos tipos de sustrato para S. macrophylla, se evaluó el número y longitud de brotes sanos. El primer sustrato consistió en una mezcla de tierra negra y agrolita con una proporción de 2:1, respectivamente (S1); el segundo sustrato se consideró el control ya que consistió en sedimentos de la Ciénega del Rio Lerma (S2). Para evaluar un método de desinfección adecuado se estableció un diseño de bloques completos al azar en donde los tratamientos consistieron en: lavados+ etanol al 70%+ cloro comercial (T1) y lavados+ microdin-jabón líquido+ etanol al 70%+ cloro comercial (T2). Las condiciones de fotoperiodo (luz y oscuridad) representaron los bloques del diseño experimental. Resultados: En el experimento de tipo de sustrato se obtuvieron diferencias estadísticas significativas (P<0.05) entre los tratamientos en donde S1 mostro 2.4±0.24 brotes en promedio con una longitud de 2.5 cm en 30 días y con tendencia a incrementar. En el experimento de desinfección se observaron diferencias estadísticas significativas (P<0.05), sin embargo el fotoperiodo no presento diferencias significativas (P>0.05). El T2 presento el menor porcentaje de contaminación 31.25%. Limitaciones del estudio/implicaciones: los resultados presentados son avances de un experimento a largo plazo. Hallazgos/conclusiones: El acondicionamiento de plantas madre de S. macrophylla es favorable en condiciones de invernadero en un sustrato compuesto por tierra negra y agrolita 2:1 permitiendo brotes nuevos y sanos. El mejor método de desinfección consistió en un enjuague a chorro de agua con jabón en polvo, 30 minutos con 100 ml de agua destilada más una gota de microdin comercial y dos de jabón líquido, dos minutos en agitación constante en etanol al 70% y 20 minutos en cloro comercial (6%) al 30% v/v, utilizando como explantes las yemas axilares de los tubérculos sin importar el fotoperiodo en el cuarto de incubación.
Objective: The objective of this work was to analyze the viability and germination of Dichromanthus aurantiacus seeds, a terrestrial orchid from Toluca valley, México. Design/methodology/approach: The size and color were evaluated. Two methods determined the viability: 1) the tetrazolium test (imbibition for 24 hours in the water, 2 hours in calcium hypochlorite (CaCOCl2), and drops of Tween-80). 2) the asymbiotic seed germination by in vitro culture (imbibition for 24 hours in the water, and the concentration of MS medium plus natural extracts). Results: The seeds of this specie showed approximately 0.2 mm long and 0.05 mm wide; they possess an embryo and a brownish testa. There were significant differences (P<0.05) between the treatments finding a positive effect with the tetrazolium test, achieving up to 91.4% viability. In the in vitro germination, the imbibition of the seeds favored contamination. The concentration of MS and the addition of natural extract presented significant differences (P<0.05), the 50% MS plus 10% of coconut water showed up to 92.8% of germination at 60 days. Study limitations/implications: The results are preliminary of a long-term experiment. Findings / Conclusions: The seeds of Dichromanthus aurantiacus showed brown testa and an oval embryo with dimensions of 0.2 mm long and 0.05 mm wide. The tetrazolium test’s viability showed 91.4% viability when they were soaked in sodium hypochlorite solution (CaCOCl2) for two hours, 24 hours soaking in tetrazolium solution (1%) plus two drops of Tween-80. The asymbiotic in vitro culture showed up to 92.8% germination in 60 days using MS medium at 50% enriched with 10% coconut water
Objective: The objective of this study was to establish the method of propagation of Oryganum vulgare and Lippia graveolens employing a plant tissue culture technique that decreased the phenolization percentages and increased the multiplication coefficients. Design/ methodology/ approach: The in vitro germination percentage was evaluated in both MS and MS medium + activated carbon. Microcuttings (small shoots) of both species were established in base medium added with different antioxidant agents to decrease the phenolization of explants; the treatments were arranged in a completely randomized block design. For the propagation phase, a completely randomized factorial design was used, where the auxin/cytokinin phytoregulators, type of explants (axillary buds and leaves), and the species (Lippia graveolens and Oryganum vulgare) were considered as factors. Results: maximum germination (63.3% ±12.5) was obtained on day 15 in both culture media for L. graveolens and O. vulgare. The use of antioxidant agents mainly activated carbon, increased the in vitro establishment and activation of vegetative buds in both species by up to 90%. There were significant differences in the variables evaluated regarding the treatments, the explant, and the species in the multiplication phase. The combination 1.0/ 0.5 mg L-1 BA/AIB induces callus formation for both species. When used as leaf explants, callus formation was potentiated. Study Limitations / Implications: The results presented are advances from a long-term experiment. Findings/conclusions: The germination of L. graveolens seeds can be achieved in MS medium after 15 days. Microcuttings of both L. graveolens and O. vulgare were successfully established in MS basal medium enriched with 1 g L-1 charcoal that showed low oxidation percentages and induced up to 90% the production of shoots in the explants. The mixture of 1.0/0.5 mg L-1 BA/AIB induces callus formation for both species; when this medium is in contact with leaves as an explant, its formation is potentiated, achieving diameters up to 15 mm. In order to achieve the induction of shoots and roots, buds should be established in MS medium enriched with 0.5 mg L-1 IBA for both species; this mixture encreased the multiplication coefficients
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