Several vegetation indices (VI) derived from handheld spectroradiometer reflectance data in the visible spectral region were tested for modelling grapevine water status estimated by the predawn leaf water potential (Ψpd). The experimental trial was carried out in a vineyard in Douro wine region, Portugal. A statistical approach was used to evaluate which VI and which combination of wavelengths per VI allows the best correlation between VIs and Ψpd. A linear regression was defined using a parameterization dataset. The correlation analysis between Ψpd and the VIs computed with the standard formulation showed relatively poor results, with values for squared Pearson correlation coefficient (r 2 ) smaller than 0.67. However, the results of r 2 highly improved for all VIs when computed with the selected best combination of wavelengths (optimal VIs). The optimal Visible Atmospherically Resistant Index (VARI) and Normalized Difference Greenness Vegetation Index (NDGI) showed the higher r 2 and stability index results. The equations obtained through the regression between measured Ψpd (Ψpd_obs) and optimal VARI and between Ψpd_obs and optimal NDGI when using the parameterization dataset were adopted for predicting Ψpd using a testing dataset. The comparison of Ψpd_obs with Ψpd predicted based on VARI led to R 2 = 0.79 and a regression coefficient b = 0.96. Similar R 2 was achieved for the prediction based on NDGI, but b was smaller (b = 0.93). Results obtained allow the future use of optimal VARI and NDGI for estimating Ψpd, supporting vineyards irrigation management.

BackgroundBacterial spot-causing xanthomonads (BSX) are quarantine phytopathogenic bacteria responsible for heavy losses in tomato and pepper production. Despite the research on improved plant spraying methods and resistant cultivars, the use of healthy plant material is still considered as the most effective bacterial spot control measure. Therefore, rapid and efficient detection methods are crucial for an early detection of these phytopathogens.MethodologyIn this work, we selected and validated novel DNA markers for reliable detection of the BSX Xanthomonas euvesicatoria (Xeu). Xeu-specific DNA regions were selected using two online applications, CUPID and Insignia. Furthermore, to facilitate the selection of putative DNA markers, a customized C program was designed to retrieve the regions outputted by both databases. The in silico validation was further extended in order to provide an insight on the origin of these Xeu-specific regions by assessing chromosomal location, GC content, codon usage and synteny analyses. Primer-pairs were designed for amplification of those regions and the PCR validation assays showed that most primers allowed for positive amplification with different Xeu strains. The obtained amplicons were labeled and used as probes in dot blot assays, which allowed testing the probes against a collection of 12 non-BSX Xanthomonas and 23 other phytopathogenic bacteria. These assays confirmed the specificity of the selected DNA markers. Finally, we designed and tested a duplex PCR assay and an inverted dot blot platform for culture-independent detection of Xeu in infected plants.SignificanceThis study details a selection strategy able to provide a large number of Xeu-specific DNA markers. As demonstrated, the selected markers can detect Xeu in infected plants both by PCR and by hybridization-based assays coupled with automatic data analysis. Furthermore, this work is a contribution to implement more efficient DNA-based methods of bacterial diagnostics.

PhenoSat is an experimental software tool that produces phenological information from satellite vegetation index time series. The main characteristics and functionalities of the PhenoSat tool are presented, and its performance is compared against observed measures and other available software applications. A multiyear experiment was carried out for different vegetation types: vineyard, low shrublands, and seminatural meadows. Temporal satellite normalized difference vegetation index (NDVI) data provided by MODerate resolution Imaging Spectroradiometer and Satellite Pour l'Observation de la Terre VEGETATION were used to test the ability of the software in extracting vegetation dynamics information. Three important PhenoSat features were analyzed: extraction of the main growing season information, estimation of double growth season parameters, and the advantage of selecting a temporal region of interest. Seven noise reduction filters were applied: cubic smoothing splines, polynomial curve fitting, Fourier series, Gaussian models, piecewise logistic, Savitzky-Golay (SG), and a combination of the last two. The results showed that PhenoSat is a useful tool to extract NDVI metrics related to vegetation dynamics, obtaining high significant correlations between observed and estimated parameters for most of the phenological stages and vegetation types studied. Using the combination of SG and piecewise logistic to fit the NDVI time series, PhenoSat obtained correlations higher than 0.71, except for the seminatural meadow start of season. The selection of a temporal region of interest improved the fitting process, consequently providing more reliable phenological information.

No abstract

No abstract